Study of Alginate-Glycerin Microspheres for Release Profile of Human Serum Albumin at Different pH Values

Ahmed Salisu¹ , Zuhra Muhammad Dikko² , Ibrahim Usman Gafai³

Section:Research Paper, Product Type: Journal-Paper
Vol.11 , Issue.2 , pp.19-23, Apr-2024

Online published on Apr 30, 2024

Copyright © Ahmed Salisu, Zuhra Muhammad Dikko, Ibrahim Usman Gafai . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
In this study, alginate-glycerin microspheres encapsulated with human serum albumin (HSA) as a model protein were synthesized utilizing the ionotropic gelation process with calcium ions acting as a cross-linker. The encapsulated microspheres were then tested for release profile using the Bradford method in simulated gastric and intestinal fluids (SGF and SIF). The native alginate and the encapsulated microspheres were characterized by FTIR analysis. The results demonstrated that the encapsulated microspheres displayed the gradual release profile of HSA in the simulated gastric fluid (SGF) compared to release profile obtained in simulated intestinal fluid (SIF), that revealed cumulative release of 46 ?g/ml of the model protein over a period of 2 hours’ incubation time. Furthermore, the in cooperated glycerin molecules improved the stability and sustained the released of the HSA in simulated intestinal fluid. In conclusion, the encapsulated microspheres demonstrated promising applications as intestinal-targeted protein vehicles.

Key-Words / Index Term :
Alginate, Protein, Delivery, Glycerin, microspheres, pH-values

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