Study of Protein in Dried Blood Stain with Respect to Time for Forensic Consideration

Komal Srivastava¹ , Vaibhav Saran² , M.K .Mishra³ , Lav Kesharwani⁴ , Neha Yadav⁵

Section:Research Paper, Product Type: Isroset-Conference
Vol.5 , Issue.3 , pp.10-16, Mar-2019

Online published on Mar 30, 2019

Copyright © Komal Srivastava, Vaibhav Saran, M.K .Mishra, Lav Kesharwani, Neha Yadav . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

View this paper at   Google Scholar | DPI Digital Library

XML View     PDF Download

How to Cite this Paper

Abstract :
Blood is an important body fluid as it is made up of various cell, protein, platelets, plasma, and other solutes. Blood constitutes about 8% of a person weight and it circulates near the surface of the skin. Almost all traumas to the body, therefore, results in the shedding of blood. Its red color makes it readily apparent at crime scence, and its residues are very difficult to remove completely. So, it has a very important entity in medico legal practices. The present study was carried out at Sam Higginbottom University and Technology Sciences and SRK pathology lab. In the present study unknown Blood samples were collected from the SRK pathology lab and chiffon clothes were also been used. The purpose of this study was for the identification of different type of protein present in the unknown blood sample for their individualization. The blood recovered at the scene of crime is generally in dried state. Blood is most frequently observed biological evidence at the crime scene pertaining to murder, sexual assault, and accidental cases. So it is very necessary to examine blood which assists the forensic analyst in an enhanced way.

Key-Words / Index Term :
Blood, Biological evidence, dried state, forensic science

References :
[1]. Adair et al. (2008) “studied the comparison of luminal and blue star reagents in detecting blood in soil nearly four years after deposition”. International Association of bloodstain pattern Analysts News, 24(4): 5 - 8
[2]. Bines J Patel, YK. Agrawal (2003), “Separation and trace estimation of Benzidinene and its molecular adducts using supercritical fluid chromatography”. J Chromatography B 795: 157 -65
[3]. Cox (1991), “A study of the sensitivity and specificity of four presumptive tests for blood”,Journal of forensic science, JFSCAVol.36, No 5
[4]. Culliford, B.J. and Nickolls, L.C; (1964) “The Benzidinene Test”, Journal of Forensic Sciences. JFSCA, Vol 9,No.1, Jan, pp. 175-191
[5]. Hass (2009), “Serological based method used in forensic casework.” Forensic Science International: Genetics, 3, 80-80
[6]. Hochmeister , M.N; Budowle, B; Sparks, and Eval R.,(1998) “Analysis of blood stains and other body fluids like sweat, saliva, semen etc immune – chromatographic test for identification of human blood” Journal of Forensic Science; 44 : 597 - 602
[7]. Inoue, H; Takabe, F; Iwasa, M; M aeno, Y; Seko, Y. (1992), “Species identification of blood and bloodstains by high performance liquid chromatography”. Forensic science international; 57:17-27
[8]. Kelstrup et al ; (2014) “Rapid and deep proteomes by rather sequencing on a bench top quadrutole ultra high – fields orbitrap mass spectrometer”. 14(6187 – 6195)
[9]. Kulak NA et al; (2014) “Minimal, in encapsulated proteomic sample processing applied to copy – number estimation in eukaryotic cells” nat methods 11 (3) :( 391 – 324)
[10]. Nichol J Martin, Josephine Bunch, Helen J Copper (2013) “Preparation and Mass Spectrometry Analysis” journal of society of American Society for Mass Spectrometry 24(8).
[11]. Spalding RP (2006), “The identification and characterization of blood and bloodstains” .In: James SH,Nordby JJ (eds) Forensic science: an introduction to scientific and investigative techniques, 2nd edn.CRC Press, Boca Raton, FL, P 237 – 260
[12]. Strasser , S; Zink, A; Kada, G; Hinterdorfer, P; Peschel,O; Heckl, W.M. et al. (2007), “Age determination of blood spots in forensic medicine by force spectroscopy”. Forensic Science International. 170: 8 – 14
[13]. Tobe, S.S. (2007) “A Evaluation of six presumptive test tests for blood , their specificity, sensitivity and effect on high molecular weight DNA”. Forensic Science International; 52,102-109.