In Vitro Evaluation of Anti Urolithiatic Activity of Bryophyllum Pinnatum Lam

Yogananth Nagarajan¹ , Rajesh Boopathi² , Syed Ali Mohamed Yahoob³ , Anuradha Venkatraman⁴

Section:Research Paper, Product Type: Journal-Paper
Vol.5 , Issue.8 , pp.97-102, Aug-2019

Online published on Aug 31, 2019

Copyright © Yogananth Nagarajan, Rajesh Boopathi, Syed Ali Mohamed Yahoob, Anuradha Venkatraman . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
Bryophyllum pinnatum is a perennial herb, widely used in the treatment of several conditions in folklore medicine, including Urolithiasis. The ethanol extracts of Bryophyllum pinnatum stem, leaf and root were evaluated on preliminary phytochemical analysis by using standard protocol and the analysis of antiuroliathiasis potency of the plant extracts on in vitro crystallization of calcium oxalate through analyzing nucleation, aggregation assays and extracted kidney stone weight reduction assay. Bioactive ingredient such as alkaloids, flavonoids, steroids, phenols, terpenoids and proteins were detected in most of the plant parts tested. In uroliathiasis activity, the maximum inhibition of CaOx crystal formation and its aggregation were detected in the following sequential order; leaf > stem > root. Also, the leaf extract of B. pinnatum was significantly reduced the weight of extracted CaOx kidney stone (% reduction) when compared with stem and root extracts. This study has given primary evidence for B. pinnatum as the plant which possess antiurolithiatic property.

Key-Words / Index Term :
Urolithiasis, Bryophyllum pinnatum, CaOx, Nucleation assay

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