Bioactive Profile and Hematological Effect of Methanol Leaf Extract of Newbouldia Laevis on Monosodium Glutamate Induced Toxicity in Female Albino Rats

Iteze P.N.¹ , Alo G.O.² , Chukwu E.C.³ , Iwueke A.V.⁴

Section:Research Paper, Product Type: Journal-Paper
Vol.6 , Issue.7 , pp.32-38, Jul-2020

Online published on Jul 31, 2020

Copyright © Iteze P.N., Alo G.O., Chukwu E.C., Iwueke A.V. . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
Newbouldia laevis (N. laevis) of the family Bigoniaeae, is commonly called boundary tree grown as an ornamental where it is believed to be the tree of fertility. This study assessed bioactive constituents and hematological effects of methanol leaf extract of Newbouldia laevis on monosodium glutamate (MSG) induced toxicity in female albino rats. Findings from proximate shows moisture, ash, fats, fibre, protein and carbohydrate contents of the sample were (mg/g): 4.67?0.02, 6.38?0.03, 1.81?0.03, 3.79?0.03, 15.77 ? 0.05, and 67.59?0.06 respectively. ?Phytochemical screening revealed the presence of (mg/g): tannins (185.30?0.03),glycosides (14.53?0.03), alkaloids (42.16?0.03), flavonoids (360.8?0.03), hydrogen cyanide (0.22?0.03), phenol (2232.27?0.03), terpenoid (994.48?0.0),steroids (2.46?0.05) and saponins (2.65?0.03). The red blood cell, haemoglobin and packed cell volume (PCV) did not differ significantly (p>0.05) among the groups. The erythrocyte sedimentation rates of rats exposed to 8000mg of MSG + 400mg/kgbw and normal saline, had significant (p<0.05) elevations relative to the MSG control, while others were insignificant (p >0.05).The platelet count of other groups were not significant (P<0.05) from the group exposed to MSG. White blood cell counts of rats exposed to 8000mg of MSG + 200mg and 400mg/kgbw of the extract were significant (p<0.05) from the MSG-alone while the mean levels of the same for those of MSG + 600mg of extract and normal saline did not differ significantly (p>0.05), There was a dose-dependent elevation of neutrophil cells in all the groups relative to the MSG alone except those of MSG + 400mg/kgbw of extract. The variation of lymphocytes and monocytes was irregular, but there was a dose-dependent lowering of eosinophils and basophils. In this study, methanol extract of N. laevis had slight effect on MSG intoxication in the rats.

Key-Words / Index Term :
Bioactive, Newbouldia laevis, hematological

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