Isolation, Characterization and Efficiency Study of Phosphate Solubilising Microorganisms from the Rock Samples of Western Ghats

I. Ameena¹ , P. Aswaja² , S. Anjana³ , C.S. Anumol⁴ , M. Anila⁵

Section:Research Paper, Product Type: Journal-Paper
Vol.6 , Issue.9 , pp.22-28, Sep-2020

Online published on Sep 30, 2020

Copyright © I. Ameena , P. Aswaja, S. Anjana, C.S. Anumol, M. Anila . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
The current study focuses on isolation, identification and characterisation of phosphate solubilizing microorganisms (PSM) from rock samples of Western Ghats. PSMs were isolated on Sperberg’s Hydroxyapatite Media (Soil Extract Agar) and Phosphate Solubilisation Efficiency (PSE) was determined on NBRIP agar and Pikovskaya’s Agar. A total of six bacterial species (PSB I, III, VI, V, VI, VII) and two fungal species were selected for further studies. The strains were characterized based on the P solubilisation zone in solid medium, and their PSE was determined on different incubation temperatures related to pH change of the medium which corresponds to the phosphatase activity, organic acids production and available phosphorus. Each isolate exhibited different results on various parameters studied. Among the six strains, PSB VII was found to be the superior in clear zone formation with PSE values 3.36S.E and 3.7S.E in Pikovaskaya’s medium and NBRIP agar respectively, whereas PSB IV showed the maximum P solubilisation efficiency quantified as 6.0SE in NBRIP agar at 320C. It can also be seen that Phosphate solubilisation by the isolated microorganisms is much higher at 32°C than at other conditions provided. The fungi solubilize P in maximum amount, was identified as Pencillum sp and Aspergillus sp. The bacterial species identified up to genus level using biochemical identification scheme and are found to be Bacillus subtilis, Comomonas sp, Pseudomonas sp, B. cereus and Arthrobacter sp. The PSMs isolated in the present study may be exploited commercially in agriculture sector. A further study on the determination of phosphatase activity and their use in plant growth promoting is underway.

Key-Words / Index Term :
Phosphorus, Western Ghats, Pikovaskaya’s medium, Sperberg’s Hydroxyapatite medium, NBRIP Agar, PSE

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