Antibacterial Activity of Phlomis Floccosa and Marrubium Alysson on Growth of Some Pathogenic Bacteria

H.I. IL Fergani¹ , M.A. Alteerah² , Y.M. Adim³ , M.M. Alareefi⁴ , E.M. Elshikhi⁵

Section:Research Paper, Product Type: Journal-Paper
Vol.6 , Issue.10 , pp.14-18, Oct-2020

Online published on Oct 31, 2020

Copyright © H.I. IL Fergani, M.A. Alteerah, Y.M. Adim, M.M. Alareefi, E.M. Elshikhi . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
The inhibitory effect of an aqueous and a methanolic extract of Phlomis floccosa leaves and vegetative parts of Marrubium alysson were tested on growth of some pathogenic bacterial strains: Klebsiella pneumonia, Escherichia coli, Streptococcus pyogenes and Staphylococcus aureus. After incubation for 24 hours at 37 °C, using a well diffusion method and comparing their inhibitory effect with the capacity of many antibiotics: Ampicillin, Erythromycin, Amoikacin and Amikacin. The alcoholic extract of Phlomis floccose showed remarkable inhibition on 3 species of bacteria, and the efficacy increased by increasing of concentrations. At 100% concentration showed the highest inhibitory capacity on each St. pyogenes, S. aureus, and Kl. Pneumonia with no inhibiting effect on E. coli. The alcoholic extract of Marrubium alysson showed remarkable inhibition activity against both St. pyogenes & S. aureus with no inhibitory effect on K. pneumonia & E. coli. The both plants aqueous extract did not show any inhibition effect on bacteria under study. The minimum inhibition concentration of the methanol extract on St. pyogenes was at 25%, on S. aureus was at 50%, on K. Pneumonia was at 100%, while there was no inhibition effect on E. coli. The antimicrobial activity of Ph. floccosa exceeded the antibiotic effect of AK 30mcg, AM 10mcg and Ax 20 mcg on S. aureus, while E 30 mcg exceeded plant extracts activity for the same bacteria. AK 30mcg effected highly on E. coli, while the highest concentration of M. alysson extract exceeded E 30mcg activity on St. pyogenes. This study result indicated to the rich valuable medicine compound in Marrubium alysson and Phlomis floccosa which have inhibitory impact on pathogenic bacteria.

Key-Words / Index Term :
The inhibitory effect of an aqueous and a methanolic extract of Phlomis floccosa leaves and vegetative parts of Marrubium alysson were tested on growth of some pathogenic bacterial strains: Klebsiella pneumonia, Escherichia coli, Streptococcus pyogenes and Staphylococcus aureus. After incubation for 24 hours at 37 °C, using a well diffusion metho

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