Antibacterial In-Vitro Evaluation of Phenotypically Screened Solasodine from Solanum nigrum Linn. Against Enterohemorrhagic Escherichia coli (0157:H7)

Michael B. Bibon¹

Section:Research Paper, Product Type: Journal-Paper
Vol.7 , Issue.8 , pp.43-48, Aug-2021

Online published on Aug 31, 2021

Copyright © Michael B. Bibon . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
Many strains of Escherichia coli (E.coli) are not detrimental yet some can cause serious illness to humans. One virulent strain of this bacterium is 0157:H7 otherwise called as the Enterohemorrhagic E.coli. Since E.coli strains cannot be treated with the available synthetic antibacterial medicines due to its recorded increasing resistance, the quest for using alternative medicines has flooded the literature to treat E.coli-related infections. This study aimed to evaluate the antibacterial efficacy of phenotypically screened solasodine from Solanum nigrum Linn. through in vitro assay against E.coli 0157:H7. Disc diffusion method was employed for the antibacterial testing of isolated 97% ± 0.18% concentration of solasodine (experimental) in comparison to the 100% commercialized solasodine (positive control), and disc treated with 80% dried methanol (negative control). Results revealed statistical no difference (p>0.05) in the measure of zone of inhibitions between the positive control and experimental set-up across trials. The result is further supported by the consistency of data across trials (p<0.05), and no appearance of zone of inhibition in the negative control set-up. The in vitro testified for the antibacterial effectiveness of solasodine isolated from Solanum nigrum Linn. extract as an alternative organic source for combating infections caused by E.coli 0157:H7. Nevertheless, further analysis of Solanum nigrum Linn. extract has to be studied to determine its effect in other tissues through in vivo assay.

Key-Words / Index Term :
Alternative medicine, Antibacterial testing, Escherichia coli, Phenotypic Screening, Phytopharmacology, Solasodine

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