Investigating the Effect of Exercise on Extracellular Vesicles-Induced Muscle Cell Proliferation Using RNA Sequencing

Catherine A. Ohiemi¹ , Ryan C. Pink²

Section:Research Paper, Product Type: Journal-Paper
Vol.8 , Issue.2 , pp.41-46, Feb-2022

Online published on Feb 28, 2022

Copyright © Catherine A. Ohiemi, Ryan C. Pink . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
Consistent physical exercise is beneficial to systemic health, and thus averts many age-related degenerative diseases. Exercise stimulates the release of molecules into the circulation, supporting the theory that inter-tissue signaling proteins are essential mediators of adaptations to exercise. Also, muscle cell-derived Extracellular Vesicles (EVs) are identified as powerful mediators of cellular differentiation, proliferation, and metabolism. This study is focused on the use of RNA Sequencing to explain the effect of exercise on extracellular vesicles-induced muscle cell proliferation. Selected persons were moderately exercised on a stationary bike for 30 minutes. Their blood samples were collected before and 30 minutes after the end of the intervention. Collection of EVs from the participants’ blood sample was done in phosphate buffered saline (PBS) from Sigma Aldrich. The total number of participants recruited for the study that generated the RNA sequence data comprised of 3 males and 3 females between 20 – 40 years. Result shows that a total number of 2618 genes were annotated. Further annotation of the genes revealed nine candidate genes involved in proliferation. It was seen that the Neb gene which is largely present in muscle cells and accounts for its structural and functional purpose had a 25 fold change in dataset and thus made it an interesting gene to look into since there has been no report of its involvement in cell proliferation. Having identified the NEB gene and with the decision to explore further, it was discovered that Exon 1 was greatly expressed in the RNAseq data.

Key-Words / Index Term :
Cellular, EVs, gene, molecular, physiological, RNA-seq

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