Telfairia Occidentalis Supplementation Attenuate Carbon-Tetrachloride-Induced Hepatic Damage and Oxidative Stress

Usunobun Usunomena¹ , Augustine Okpiabhele² , Ademuyiwa J. Adegbegi³

Section:Research Paper, Product Type: Journal-Paper
Vol.9 , Issue.5 , pp.69-76, May-2023

Online published on May 31, 2023

Copyright © Usunobun Usunomena, Augustine Okpiabhele, Ademuyiwa J. Adegbegi . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
This study determined hepatoprotective effect of Telfairia occidentalis aqueous leaf extract on Carbon-tetrachloride (CCl4)-induced toxicity in male Wistar rats. Of the five experimental groups presented, first group was control and it received distilled water. Rats in second group received CCl4 alone for four consecutive days. Rats in third and fourth groups were administered CCl4 for four consecutive days prior to treatment with 200mg/kg and 400mg/kg T. occidentalis aqueous extract for six consecutive days respectively. The fifth group received CCl4 for four consecutive days prior to treatment with Silymarin (100 mg/kg) for six consecutive days. All rats intraperitoneally received 1ml/kg, 1:1, a mixture of freshly prepared CCl4 in olive oil with exception of normal control rats. Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT), Alkaline Phosphatase (ALP), Lactate Dehydrogenase (LDH), Acid Phosphatase (ACP) were assayed in the serum while Superoxide Dismutase (SOD), Catalase (CAT), Malondialdehyde (MDA), and Nitric Oxide (NO) in the liver homogenates were measured. From results obtained, exposure to CCl4 orchestrated significant (p ? 0.05) increases in AST, ALT, ALP, LDH, ACP, MDA and NO and significantly (p ? 0.05) decreased SOD and CAT whereas T. occidentalis aqueous extract administration at a dose of 200mg/kg and 400mg/kg alleviated toxic effects of CCl4. The result of toxicity of CCl4 and hepatoprotection by T. occidentalis were corroborated by histopathological analysis. In conclusion, this study established that T. occidentalis possesses and enhanced antioxidant activity, thus attenuating CCl4-induced hepatic oxidative damage and oxidative stress.

Key-Words / Index Term :
Antioxidants, Carbon-tetrachloride, Hepatotoxicity, Liver, Oxidative stress, Telfairia occidentalis

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