Production, Purification and efficacy determination of Epsilon toxin from Clostridium perfringens type ‘D’ IVRI native culture

Richa Tiwari¹ , Geetika Bakshi² , Niranjan Tiwari³ , Nitin Puranik⁴

Section:Research Paper, Product Type: Isroset-Journal
Vol.2 , Issue.2 , pp.1-4, Mar-2015

Online published on Nov 15, 2015

Copyright © Richa Tiwari, Geetika Bakshi, Niranjan Tiwari , Nitin Puranik . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
Epsilon toxin of C.perfringens type ‘D’ IVRI native strain was produced by Batch type flask culture and further purified by using APS salt precipitation, Dialysis, DEAE sepharose separation and SDS PAGE analysis. It was also subjected to determine the toxicity by sing mouse toxicity test. Purified toxin showed the potent toxicity even at lowest dilution of 1:10,00,000 (toxin:Phosphate buffered saline).These findings suggested that purification increases the potency of toxin and thus lower the need of dose of vaccine administration which produced by the conversion of this toxin to toxoid. After purification a band of 32 kDA was obtained in SDS acrylamide gel autoradiograph showed the homogeneity with previously obtained results in this area of work. Epsilon toxin can penetrate the blood brain barrier so it could be prove as a potent vehicle for drug delivery system.

Key-Words / Index Term :
C.perfringens type ‘D’, Enterotoxaemia, Epsilon toxin, DEAE sepharose purification, Mouse toxicity test

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