Isolation and molecular characterization of Enterobacter species ENKS2 and its phosphate solubilisation from rhizospheric soil

Santhanalakshmi Krishnamoorthy¹ , Natarajan Ekambaram²

Section:Research Paper, Product Type: Isroset-Journal
Vol.5 , Issue.6 , pp.20-25, Dec-2018

CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v5i6.2025

Online published on Jan 01, 2018

Copyright © Santhanalakshmi Krishnamoorthy, Natarajan Ekambaram . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
In this present study, the potential of a phosphate solubilising bacterium (PSB) was isolated from rhizosphere soil. The phosphate solubilisation was grown in Pikovskaya’s agar medium and the isolate was purified in AT salt minimal medium and further it was cultured in NBRIP liquid media for characterization, identification processes. Based on the morphological, biochemical characterization and 16S rRNA gene sequencing the phosphate solubilising bacterium was identified as Enterobacter species ENKS2. The maximum phosphate solubilisation was in 96h at pH 7.3 in NBRIP liquid media. The Enterobacter species ENKS2 optimized phosphate solubilisation was at pH 7 (281µg/cfuX106), temperature at 30°C (268µg/cfuX106) at pH 6.8, salt conditions was 0.5% (169µg/cfuX106) at pH 7.1, carbon source xylose (213µg/cfuX106) at pH 6.3 and nitrogen source Pottassium nitrate (212µg/cfuX106) at pH 6.1. The different time intervals were followed to maximum phosphate solubilisation in 96h (531µg/cfuX106) at pH 5.8. Hence, this study was useful to develop the biofertilizer for improves the soil, plant growth and reduce the chemical fertilizer usages.

Key-Words / Index Term :
Phosphate solubilisation, Rhizospheric soil, Enterobacter sp., 16S rRNA gene sequencing, acid phosphates

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