In Saccharomyces cerevisiae the attenuation of the 2- deoxy-D-glucose toxicity is alleviated by inositol

Chidambaram Ravi¹ , Vasanthi Nachiappan²

Section:Research Paper, Product Type: Isroset-Journal
Vol.5 , Issue.6 , pp.56-60, Dec-2018

CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v5i6.5660

Online published on Jan 01, 2018

Copyright © Chidambaram Ravi, Vasanthi Nachiappan . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
In Saccharomysis cerevasiae the glycolytic transcription factor GCR1 tightly controls the expression of genes involved in glycolysis but the importance of Gcr1 transcription in 2-DG toxicity is still elusive. In the present study, we observed 2-DG toxicity in the wild-type, gcr1∆, and ino2∆ cells under inositol absence (I−) condition obstruct the growth, hence the opi1∆ cells were resistant to 2-DG exposure. Further, the presence of 2-DG significantly decreased the promoter activity of INO1 in the WT cells. The 2-DG exposure suppressed the growth rate in WT and gcr1∆ strains under inositol limitation condition, but the opi1∆ (over production of inositol) strain was resistant to the 2-DG exposure with I− condition. Taken together, the above findings suggest that 2-DG exposure reduced the promoter activity of INO1 leads to inositol auxotrophic growth defect, hence the inositol supplementation bring back normal. To conclude that the inositol alleviates the 2-DG toxicity in Saccharomyces cerevisiae.

Key-Words / Index Term :
2-deoxy-D-glucose, INO1, β-Galactosidase activity, over production of inositol

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