Volume-5 , Issue-3 , Jun 2018, ISSN 2347-7520 (Online) Go Back

• Open Access   Article

  Evaluation of immunomodulatory effect of Tinospora cordifolia leaf and stem on zidovudine induced immunotoxicity

  G. Nageswari, A. Harinatha Reddy, B. Venkatappa

  Research Paper | Isroset-Journal (IJSRBS)

  Vol.5 , Issue.3 , pp.1-5, Jun-2018

  CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v5i3.15

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  Abstract
  The present study was conducted to evaluate immunomodulatory effect of Tinospora cordifolia (T.C) leaf and stem extracts on zidovudine induced immunotoxicity. T.C leaves and stem was collected and three different extracts of stem and leaf (Aqueous, methanol, n-hexane) was prepared by Soxhlet extraction. Total 24 rats were used in the study. Rats were divided into 4 groups each of 6 rats. Group-I was treated with distilled water. Group-II rats received Zidovudine (500 mg/kg), Group-III given alcoholic extract of T.C stem (500 mg/kg) + Zidovudine (500 mg/kg) and Group-IV received alcoholic extract of T.C leaf (500 mg/kg) + Zidovudine (500 mg/kg). Physical and hematological and immunological parameters were measured at every alternative week. At the end of study rats were sacrificed under anesthesia. Thymus and spleen was collected and weighed. These findings suggest ameliorate effect of T.C leaf and stem extract on zidovudine induced immunotoxicity.

  Key-Words / Index Term
  Zidovudine, alcoholic extract, immunomodulatory, immunotoxicity, haematological and histopathological

  References
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  Citation
  G. Nageswari, A. Harinatha Reddy, B. Venkatappa, "Evaluation of immunomodulatory effect of Tinospora cordifolia leaf and stem on zidovudine induced immunotoxicity," International Journal of Scientific Research in Biological Sciences, Vol.5, Issue.3, pp.1-5, 2018

• Open Access   Article

  Evaluation of efficacy of collection techniques for human genomic DNA: MAOA-uVNTR polymorphism

  M.S. Siva Prasad and Y Shibu Vardhanan

  Research Paper | Isroset-Journal (IJSRBS)

  Vol.5 , Issue.3 , pp.6-11, Jun-2018

  CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v5i3.611

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  Abstract
  For large scale DNA related studies it is necessary for non-invasive and cost-effective method for obtaining sufficient quantity and quality of DNA. In this study we collected buccal cells, dried blood and saliva from 10 participants by commercially available two types of foam swabs (Puritan and HiMedia), blood collection card (NucleoSave) and saliva collection kit (Oragene OG-500) respectively. Genomic DNA was extracted from the seven days old buccal cells and dried blood using QIAamp DNA Mini Kit (Qiagen) with a modification in the final elution step which resulted in high yield of DNA. Oragene DNA extraction protocol was followed to extract DNA from saliva. Integrity, yield and purity of extracted DNA were compared by agarose gel electrophoresis, spectrophotometry and PCR amplification success for specific gene. Though, the DNA yield was significantly greater from samples of saliva than buccal cells and dried blood, contamination was more in DNA from saliva. Purity of DNA obtained from buccal cells collected by Puritan foam swabs was better for the PCR reaction as evidenced by the successful amplification of 30-bp repeat polymorphism in the promoter of the MAOA gene. Hence, we conclude that Puritan foam swab is an ample and cost-effective method for collecting buccal cells for extracting genomic DNA for epidemiological and forensic studies.

  Key-Words / Index Term
  Saliva, buccal cell, dried blood, genomic DNA, PCR

  References
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  Citation
  M.S. Siva Prasad and Y Shibu Vardhanan, "Evaluation of efficacy of collection techniques for human genomic DNA: MAOA-uVNTR polymorphism," International Journal of Scientific Research in Biological Sciences, Vol.5, Issue.3, pp.6-11, 2018

• Open Access   Article

  Assessment of Hormone Levels in Men with Fertility Problems Attending a Tertiary Infertility Center in North Karnataka

  M. M. Toragall, S. K. Satapathy, G. G. Kadadevaru, M. B. Hiremath

  Research Paper | Isroset-Journal (IJSRBS)

  Vol.5 , Issue.3 , pp.12-15, Jun-2018

  CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v5i3.1215

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  Abstract
  we assessed the serum levels of Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH) and Testosterone (T) which are the principle hormones responsible for the control of spermatogenesis. A total of 125 married men facing fertility problem and 35 proven fathers were assessed for the serum hormone levels and the sperm count, attending a tertiary infertility center in North Karnataka. Statistical analysis of different variables were performed using SPSS 20.0. Significant reduction in mean sperm count and increased Luteinizing Hormone levels among cases was found (p <0.05). There was an insignificant difference between the mean serum levels of Follicle Stimulating Hormone and Testosterone of the cases to that of the control group (p = 0.191, p = 0.228). The current study found significant correlation of Sperm count with serum Follicle stimulating hormone and (p = 0.014) and insignificant correlation with Luteinizing hormone (p = 0.523) and Testosterone (p = -0.078) among cases.

  Key-Words / Index Term
  Spermatogenesis, Male Infertility, Follicle Stimulating Hormone, Luteinizing Hormone, Testosterone

  References
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  Citation
  M. M. Toragall, S. K. Satapathy, G. G. Kadadevaru, M. B. Hiremath, "Assessment of Hormone Levels in Men with Fertility Problems Attending a Tertiary Infertility Center in North Karnataka," International Journal of Scientific Research in Biological Sciences, Vol.5, Issue.3, pp.12-15, 2018

• Open Access   Article

  Adsorption of Lead Using Pogamia Pinata Powder as A Low Cost Adsorbent

  Vijay R. Ram, Jiva P. Odedra, Suhas J Vyas, Savan P. Tank

  Research Paper | Journal-Paper (IJSRBS)

  Vol.5 , Issue.3 , pp.16-20, Jun-2018

  CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v5i3.1620

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  Abstract
  The study on performance of low-cost adsorbent such as pinata leaves powder in the removal of Lead (II) ion from aqueous solution is performed. In present study p. piñata Leaves Powder adsorbent prepared. The adsorbent material adopted was found to be an efficient media for removal of Lead (II) ion with different parameter like Adsorbent dosages, Concentration and contact time etc. The different contact time and reduced concentration determines using Atomic Absorption Spectrophotometer (AAS). It was found that the metal uptake capacity (amount of removal) of and Lead (II) ion decreased but the adsorption capacity (percentage of removal) increased with the decrease in the concentration of and Lead (II) in the initial sample solution.

  Key-Words / Index Term
  Low cost, Piñata leave, AAS, Ion decrease, Lead

  References
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  Citation
  Vijay R. Ram, Jiva P. Odedra, Suhas J Vyas, Savan P. Tank, "Adsorption of Lead Using Pogamia Pinata Powder as A Low Cost Adsorbent," International Journal of Scientific Research in Biological Sciences, Vol.5, Issue.3, pp.16-20, 2018

• Open Access   Article

  Antifungal activity of Streptomyces longisporoflavus isolated from Lonar Lake against Alternaria solani

  H.J. Bhosale, S. Raut., T.A. Kadam

  Research Paper | Isroset-Journal (IJSRBS)

  Vol.5 , Issue.3 , pp.21-26, Jun-2018

  CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v5i3.2126

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  Abstract
  Actinomycetes were isolated from water samples of Lonar Lake, Buldhana district of Maharashtra, India and screened for their antifungal activity by agar well diffusion method against phyto-pathogenic fungus Alternaria solani. The strain LL-5 showing highest antifungal activity was identified as Streptomyces longisporoflavus on the basis of microscopic and biochemical characteristics. The antifungal metabolite production using S. longisporoflavus was optimized based on optimal incubation period, temperature, initial pH of medium and supplementation of carbon and nitrogen sources. The study revealed that incubation period of 8 days, 30ᴼC, pH 10.0, starch (1%) as carbon source and peptone (0.5%) as nitrogen source are favorable parameters for maximum production of antifungal metabolite by S. longisporoflavus. These results clearly elucidates that S. longisporoflavus isolated from Lonar Lake water sample could be a good source of antifungal metabolite that can be used for management of diseases caused by Alternaria solani.

  Key-Words / Index Term
  Antifungal activity, Alternaria solani, Lonar Lake, Streptomycetes, disease management.

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  Citation
  H.J. Bhosale, S. Raut., T.A. Kadam, "Antifungal activity of Streptomyces longisporoflavus isolated from Lonar Lake against Alternaria solani," International Journal of Scientific Research in Biological Sciences, Vol.5, Issue.3, pp.21-26, 2018

• Open Access   Article

  Variability Studies of POD and Seed Characterstics of Albizia Procera in Chhattisgarh

  Sunil Kumar Tiwari and S S Dhuria

  Research Paper | Isroset-Journal (IJSRBS)

  Vol.5 , Issue.3 , pp.27-31, Jun-2018

  CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v5i3.2731

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  Abstract
  Albizia procera is an important agroforestry tree species widely distributed across different agricultural systems of India.. Therefore to identify the best seed source is a big challenge and to understand about the seed morphology, germination and seedling growth of some provenances/ seedsource is an important step in helping identification of suitable provenance for establishment in Chhattisgarh. The present study was conducted in Departmental Nursery to determine the variation in pod and seed characteristics in five provenances namely Bilaspur, Bastar, Korba, Raigarh and Sarguja of Chhattisgarh,India. The studies conducted through the estimation of range, mean, phenotypic and genotypic coefficient of variability, heritability, genetic advance and genetic gain. The present study revealed that highly significant differences among provenances in all studied parameters except pod thickness. Highest variation among provenances in seed traits were observed in seed weight and seed length while seed thickness has the lowest variation. Significant variations were recorded in all the studied parameter. The extent of variation in pod length and seed width was higher as compared to pod width, seed thickness, seed length and no. of seeds per pod, high heritability is recorded in pod length(46.01%), seed width (67.74%), seed thickness (45.00%) so there is scope for considerable genetic gain.

  Key-Words / Index Term
  Variability, seed characteristics, genetic gain, Albizia procera

  References
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  [10] H. S Ginwal, M. Gera and R. L. Srivastava .” Seed source variability in some seed and seedling characteristics of twenty provenances of Acacia nilotica Willd. ex. Del.” Range Management & Agroforestry 17 (1): 49-59. 1996.
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  Citation
  Sunil Kumar Tiwari and S S Dhuria, "Variability Studies of POD and Seed Characterstics of Albizia Procera in Chhattisgarh," International Journal of Scientific Research in Biological Sciences, Vol.5, Issue.3, pp.27-31, 2018

• Open Access   Article

  Emerging fungal contaminants isolated and identified from raw fennel seeds

  Chinju Merin Abraham, Tincy Mary Thomas

  Research Paper | Isroset-Journal (IJSRBS)

  Vol.5 , Issue.3 , pp.32-35, Jun-2018

  CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v5i3.3235

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  Abstract
  The present investigation entitled “Emerging fungal contaminants isolated and identified from raw fennel seeds” recorded the diversity of surface contaminant fungi from 144 fennel seeds of different A/C and non-A/C hotels of town area of Pathanamthitta district of Kerala, which were cultured on PDA medium. All fungal species were identified on the basis of their morphological and microscopic characteristics and it includes Aspergillus niger, Aspergillus fumigatus, Chrysonilia sitophila, Mucor sp., Rhizopus sp., Cercospora sp. and Cylindrocarpon sp. The study revealed its colonization frequency, counted the fungal colony numbers, determined the percentage of different fungal isolates and finally compared the A/C and non-A/C seed contaminants. And from the study, it was clear that samples from non-A/C hotels shows maximum fungal growth than A/C hotels. These emerging fungal contaminants can cause serious pathogenic conditions such as aspergillosis, zygomycosis, occupational asthma etc.

  Key-Words / Index Term
  Fungal contaminants, Fennel seeds, Pathanamthitta, PDA medium, Colonization frequency, Aspergillosis

  References
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  Citation
  Chinju Merin Abraham, Tincy Mary Thomas, "Emerging fungal contaminants isolated and identified from raw fennel seeds," International Journal of Scientific Research in Biological Sciences, Vol.5, Issue.3, pp.32-35, 2018

• Open Access   Article

  Chemical Characterization of Cumin Seed Oil (Cuminum Cyminum) by Gcms and its Comparative Study

  S. R. Gotmare and E. A. Tambe

  Research Paper | Isroset-Journal (IJSRBS)

  Vol.5 , Issue.3 , pp.36-45, Jun-2018

  CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v5i3.3645

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  Abstract
  Cumin (Cuminum cyminum L.) is among the large number of spices used to flavour food and beverages. In this study the chemical composition of the extracted cumin seed oil and market cumin oil were analysed by GCMS and compared. The comparative study revealed that there is a huge difference between these two oils. The extracted cumin oil was found rich in oxygenated terpenoids. In addition to this, a comparative study from the data of chemical composition of cumin seeds from different countries also showed variation in the chemical components depending upon the geography, environment, climate, harvesting stage, etc.

  Key-Words / Index Term
  Hydrodistillation, cuminaldehyde, Iran, antioxidant, antimicrobial

  References
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  Citation
  S. R. Gotmare and E. A. Tambe, "Chemical Characterization of Cumin Seed Oil (Cuminum Cyminum) by Gcms and its Comparative Study," International Journal of Scientific Research in Biological Sciences, Vol.5, Issue.3, pp.36-45, 2018

• Open Access   Article

  Nutritional and Medicinal value of Moringa oleifera

  Amanjot Kaur

  Review Paper | Isroset-Journal (IJSRBS)

  Vol.5 , Issue.3 , pp.46-50, Jun-2018

  CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v5i3.4650

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  Abstract
  Moringa oleifera Lam is a highly valued plant which is found in many countries of the tropics and subtropics. All the parts of this tree are useful either for nutritional or Medicinal purposes. Various parts of this plant such as the leaves, roots, seed, bark, fruit, flowers and immature pods act as cardiac and circulatory stimulants, possess antitumor, anti inflammatory, antihypertensive, cholesterol lowering and antifungal activities and are being employed for the treatment of different ailments in the indigenous system of medicine, particularly in South Asia. For cooking, usually leaves, flowers and Pods are used. They possess miraculous health benefits. Bark and roots are used in traditional medicines. This tree has small leaves, white flowers and seed pods which are long, slender and triangular resembling drumsticks so this tree is also known as Drumsticks in English. Different parts of Moringa oleifera contain important minerals and are a good source of proteins, vitamins, beta-carotene, amino acids and various phenolics. Moringa oleifera provides a rich and rare combination of zeatin, quercetin, beta-sitosterol, caffeoylquinic acid and kaempferol.

  Key-Words / Index Term
  Moringa oleifera, Cardiac, Drumsticks, Phenolics, Stimulants

  References
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  [4] N.Sharma, D.S. Rathore, “Antibacterial effects of Citrus limon peel extract on human pathogenic bacteria with special reference to Urinary Tract Infection”, IJSRBS,Vol.5, Issue.2, pp. 14-17, April (2018) E-ISSN: 2347-7520
  [5] S. Sugandha, “Callus Induction, Anti Microbial Screening and in Vitro Plantlet Regeneration of Adansonia Digitata L.: Anendangered Medicinal Tree”, IJSRBS, Vol. 2 issue 5, pp. 10-16, Oct (2015) E-ISSN: 2347-7520
  [6] R. Suresh,S.Thampiraj, and A. Stephen, “Antibacterial activities of wild rhizomatous plants - Curcuma aromatica, Curcuma longa (Zingiberaceae) and synergistic effects of both collected from southern Western Ghats, India”, IJSRBS, Vol. 5 issue 2, pp. 7-13, April(2018) E-ISSN: 2347-7520
  [7] H.R. Raveesha, T. Uthpala, “In vitro Callus Induction and Preliminary Phytochemical Studies of Cissampelos pareira L”, IJSRBS, Vol. 5 issue 1, pp. 12-17, Feb (2018) E-ISSN: 2347-7520
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  Citation
  Amanjot Kaur, "Nutritional and Medicinal value of Moringa oleifera," International Journal of Scientific Research in Biological Sciences, Vol.5, Issue.3, pp.46-50, 2018

• Open Access   Article

  Preliminary Phytochemical Analysis and Invitro Anti–viral Activity of Ethanolic extract of Whole plant of Tinospora cordifolia (Thunb.) Miers against Hepatitis-A Virus

  Ramaiah Maddi, Venkata Lakshmi Kandula, Bhagyavathi Vallepu, Hymavathi Navuluri, Harshavardhan Kollu

  Research Paper | Isroset-Journal (IJSRBS)

  Vol.5 , Issue.3 , pp.51-55, Jun-2018

  CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v5i3.5155

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  Abstract
  Herbal product design refers to the process of developing, standardizing, processing, and validating an herbal product for the market. Hepatitis A is an infectious disease of the liver caused by the hepatitis A virus (HAV). There are no specific medicines to cure infection with hepatitis A. Hence, there is a need for the development of less toxic, more efficacious and cost-effective antiviral agents. The present work highlighted the invitro anti–viral activity of ethanolic extract of whole plant of Tinospora cordifolia (Thunb.) Miers against HAV. Ethanolic extract of the selected plant was prepared by soxhlet apparatus and rotavap. For the antiviral screening against HAV, toxicological effect of selected plant extract was determined through MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell proliferation assay using Huh-7 cell lines. The standard used is Camptothecin (CPT) at 25µG concentration. Plant extract was tested at 3.125 μg/mL, 6.25μg/mL,12.5μg/mL, 25 μg/mL and 50 μg/mL concentrations. The cell morphology was observed and recorded under microscope. The results clearly indicated the selected plant extract shows a dose dependent activity and it was maximum at 50 μg/mL concentration where it shows only 56.24% of virus viability and it was comparable to standard drug where it shows 43.01% of viability. Phytochemical screening of selected plant extract reveals the presence of different secondary metabolites like alkaloids, flavonoides, tannins, resins, steroids. Presence of these compounds in alone or combination might be responsible for the observed antiviral activity.

  Key-Words / Index Term
  Tinospora cordifolia; whole plant; MTT assay; Huh-7 cell lines; anti-viral

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  Citation
  Ramaiah Maddi, Venkata Lakshmi Kandula, Bhagyavathi Vallepu, Hymavathi Navuluri, Harshavardhan Kollu, "Preliminary Phytochemical Analysis and Invitro Anti–viral Activity of Ethanolic extract of Whole plant of Tinospora cordifolia (Thunb.) Miers against Hepatitis-A Virus," International Journal of Scientific Research in Biological Sciences, Vol.5, Issue.3, pp.51-55, 2018