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• Open Access   Article

  Preparation of Stable Silver Nanoparticle Containing Glycerol-AOT [bis(2-ethylhexyl)sulfosuccinate sodium salt] Reverse Micellar Solution using Hydrogen Gas as Reducing Agent and Silver Nitrate as Precursor Material

  Palash Setua

  Research Paper | Journal-Paper (IJSRBS)

  Vol.6 , Issue.5 , pp.1-4, Oct-2019

  CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6i5.14

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  The research paper describes the synthesis of silver nanoparticles in nonaqueous AOT [bis (2-ethylhexyl) sulfosuccinate sodium salt] reverse micellar solution. Complete description of the reverse micellar system is glycerol-AOT-n-heptane reverse micelle where AOT molecules act as surfactant molecule, glycerol as non aqueous polar core solvent and n-heptane as nonpolar hydrocarbon which acts as the dispersion medium. In this study I have used glycerol as nonaqueous polar solvent which was used to create the AOT surfactant core of the reverse micelle and then incorporated silver ion (Ag+) using silver nitrate (AgNO3) salt as precursor material responsible for metal nanoparticle formation. Freshly prepared hydrogen gas (H2) is used as reducing agent to reduce silver ion (Ag+) to silver zero (Ag0) state. Formation and precipitation of large particle was restricted by the cage like templating structure of reverse micelle. I have selected hydrogen gas as reducing agent because of its mild nature and also wanted to minimize the perturbation of natural reverse micelle environment

  Key-Words / Index Term
  Metal Nanoparticle, Silver, Reverse Micelle, Nonaqueous solvent medium

  References
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  [10] C. Petit, P. Lixon, M.P. Pileni, “In situ synthesis of silver nanocluster in AOT reverse micelles”, J. Phy. Chem., Vol. 97, Issue. 49, pp. 12974-12983.
  [11] P. Setua, R. Pramanik, S. Sarkar, C. Ghatak, S.K. Das, N. Sarkar, “Synthesis of silver nanoparticle inside the nonaqueous ethylene glycol reverse micelle and a comparative study to show the effect of the nanoparticle on the reverse micellar aggregates through solvation dynamics and rotational relaxation measurements” J. Phy. Chem. B, Vol. 114, Issue. 22, pp. 7557-7564.
  [12] P. Setua, R. Pramanik, S. Sarkar, C. Ghatak, V.G. Rao, N. Sarkar, S.K. Das, “Synthesis of silver nanoparticle in imidazolium and pyrolidium based ionic liquid reverse micelles: A step forward in nanostructure inorganic material in room temperature ionic liquid field”, Journal of Molecular Liquids, Vol. 162, Issue. 1, pp. 33-37.
  [13] P.Setua, A. Chakraborty, D. Seth, M.U. Bhatta, P.V. Satyam, N. Sarkar, “Synthesis, optical properties, and surface enhanced raman scattering of silver nanoparticle in nonaqueous methanol reversemicelles”, J. Phy. Chem. C, Vol. 111, No. 10, pp. 3901-3907, 2007. and references therein.
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  Citation
  Palash Setua, "Preparation of Stable Silver Nanoparticle Containing Glycerol-AOT [bis(2-ethylhexyl)sulfosuccinate sodium salt] Reverse Micellar Solution using Hydrogen Gas as Reducing Agent and Silver Nitrate as Precursor Material," International Journal of Scientific Research in Biological Sciences, Vol.6, Issue.5, pp.1-4, 2019

• Open Access   Article

  In Situ Production of Silver Bio Nanoparticles from Marine Biosurfactant Bacteria and Evaluation of Its Antibacterial Activity

  M. Indhuja, D. Kavitha, P. Selvamaleeswaran, A. Palanisamy, M. Sureshkumar

  Research Paper | Journal-Paper (IJSRBS)

  Vol.6 , Issue.5 , pp.5-11, Oct-2019

  CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6i5.511

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  Abstract
  Biosurfactants are the surface-active molecules synthesized by microorganisms. With the advantage of environmental compatibility, the demand for biosurfactants has been steadily increasing and may eventually replace their chemically synthesized counterparts. Marine biosurfactants produced by some marine microorganisms have been paid more attention, particularly for the antimicrobial activity against various microbes in medicinal field. In this study, the screening of biosurfactant-producing marine microorganisms, the determination of biosurfactant activity as well as the recovery of marine biosurfactant and the antibacterial activity of the biosurfactant were done. The uses of silver nanoparticles with marine biosurfactants for the antibacterial activity also discussed. The marine Serratia sp. had the ability to produce the biosurfactant and shows high amount of antibacterial activity against some pathogenic bacteria

  Key-Words / Index Term
  Biosurfactant, marine microorganisms, Silver nanoparticles

  References
  [1] S.K. Satpute, “Assessment of different screening methods for selecting biosurfactant producing marine bacteria”, Ind. J. Mar. Sci., Vol. 3, Issue. 7, pp. 243-250, 2008.
  [2] K. K Gautam, and V. K. Tyagi, “Microbial surfactants: A review”. J. Oleo. Sci., Vol. 55, pp.155-166, 2005.
  [3] P. C. Nagajyothi, and K.D. Lee, “Synthesis of Plant-Mediated Silver Nanoparticles Using Dioscoreabatatas Rhizome Extract and Evaluation of Their Antimicrobial Activities”, J. Nanomaterials, pp. 1-7, 2011.
  [4] I.V. Nwaguma, C.B. Chikere, and G.C. Okpokwasili, “Isolation, characterization, and application of biosurfactant by Klebsiella pneumoniae strain IVN51 isolated from hydrocarbon-polluted soil in Ogoniland, Nigeria”, Bioresour. Bioprocess, Vol.3, Issue. 40, 2016.
  [5] S. Dhail, and N.K. Jasuja, “Isolation of biosurfactant producing marinebacteria”, African journal of environmental science technology. Vol. 6, Issue. 6, pp. 263-266, 2012.
  [6] P.K.S.M. Rahman, G. Pasirayi, V. Auger, and Z. Ali, “Production of rhamnolipid biosurfactants by Pseudomonas aeruginosa DS10-129 in a microfluidic bioreactor”, Biotechnology and Applied Biochemistry, Vol. 55, Issue. 1, pp.45-52, 2010.
  [7] A. J. Das, R. Kumar, S. P. Goutam, and S. S. Sagar, “Sunlight irradiation induced synthesis of silver nanoparticles using glycolipid bio-surfactant and exploring the antibacterial activity”, Journal of Bioengineering and Biomedical Science, Vol. 6, Issue. 5, 2016.
  [8] H. S. Jaffat, N. H. Aldujaili, and A. J. Abdul Hassan, “Antimicrobial Activity of Silver Nano Particles Biosynthesized by Lactobacillus Mixtures”, Research Journal of Pharmaceutical, Biological and Chemical Sciences, Vol. 8, Issue. 1, pp. 1911-1924, 2017.
  [9] E. Ranganath, V. Rathod, and A. Banu, “Screening of Lactobacillus spp. for Mediating the Biosynthesis of Silver Nanoparticles from Silver Nitrate”, IOSR Journal of Pharmacy, Vol. 2, Issue. 2, pp. 237-241, 2012.
  [10] T. Deepa Singh, P. Sravani, S. P. Sreedhar Bhattar, and K. Anuradha, “Green synthesis of iron, copper and silver nanoparticles and their antibacterial activity on animal pathogens – A Comparative study”, Int. J. Sci. Res. in Biological Sciences, Vol. 6, Issue. 1, pp.19-24, 2019.
  [11] P. R. Chaudhari, S. A. Masurkar, V. B. Shidore, and S. P. Kamble, “Antimicrobial activity of extracellularly synthesized silver nanoparticles usingvLactobacillus species obtained from VIZYLAC Capsule”, Journal of Applied Pharmaceutical Science, Vol. 2, Issue. 3, pp. 25-29, 2012.
  

  Citation
  M. Indhuja, D. Kavitha, P. Selvamaleeswaran, A. Palanisamy, M. Sureshkumar, "In Situ Production of Silver Bio Nanoparticles from Marine Biosurfactant Bacteria and Evaluation of Its Antibacterial Activity," International Journal of Scientific Research in Biological Sciences, Vol.6, Issue.5, pp.5-11, 2019

• Open Access   Article

  The Ecology of Merops Orientalis

  Swetaleena Mishra

  Research Paper | Journal-Paper (IJSRBS)

  Vol.6 , Issue.5 , pp.12-14, Oct-2019

  CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6i5.1214

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  Abstract
  An ecosystem functions by the interrelationship between the different types of living organisms found in it. The birds are a vital component of many a food chains as they occupy diverse habitats and therefore, they play a very important role in establishing ecological balance in the nature. Thus, study of avian ecology is very important aspect of life-science. The Merops orientalis, commonly called as the ‘green bee-eater’, is a very beautiful bird which is commonly found in the countryside. This study has been conducted to know the feeding, social and nesting behaviors of these tiny green bee-eaters. These amazing insectivorous birds are commonly found in this lush green region of Mosabani, a rural area in Jharkhand. These small hunters are slender having very colorful plumage and a typical tail structure. They mostly occur in pairs and nest in colonies. They roost on trees huddled in large groups making loud shrilling ‘treee- treee’ sounds. These birds camouflage beautifully with the green leaves of the trees and bushes

  Key-Words / Index Term
  Clutch, Fledgling, Insectivorous, Nest-tunnels, Roost

  References
  [1]. A.M.S. Ali, and A. Subramanian, “Diurnal-activity patterns of the Small Bee-eater (Merops orientalis) in Southern India”. Tropical Life Sciences, Vol. 26, Issue.1, pp. 9-20, 2015.
  [2]. A.K. Banerjee, “Some observations on the breeding behaviour of Bluebearded Bee-eater and Indian Small Green Bee-eater”, Zoos’ Print Vol. 7, Issue.12, pp. 19-21, 1992.
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  [4]. B.K. Schmidt, and W.R. Branch, “Nest and eggs of the Black-headed Bee-eater (Merops breweri) in Gabbon, with note on other bee-eaters”, Ostrich, Vol. 76, Issue.1&2, pp. 80-81, 2005.
  [5]. C.H. Fry, “Family Meropidae (bee-eaters). Handbook of the birds of the world”, Vol. 6, pp. 286-341, 2001.
  [6]. C.H. Fry, K. Fry and A. Harris, “Kingfishers, Bee-eaters and Rollers”, Christopher Helm, London, 1992.
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  [9]. M.S. Mewada, “Ecological Study and Avian Faunal Diversity of Narmada River and its Surrounding Areas of Dindori District (M.P)”, International Journal of Scientific Research in Biological Sciences, Vol. 4, Issue. 1, pp. 4/9, 2017.
  [10]. R.C. Mishra, “Green Bee-eater, Merops orientalis and evaluation of possible methods to reduce honeybee predation”, Pavo Vol. 31, Issue.1-2, pp. 113-116, 1993.
  [11]. R.C. Sihag, “The green bee-eater Merops orientalis latham-(1) – Seasonal activity, population density, feeding capacity and bee capture efficiency in the apiary of honey bee, Apis mellifera L. in Haryana (India)”. Korean Journal of Apiculture, Vol. 8, Issue.1, pp. 5-9, 1993.
  [12]. S. Ali, and S.D. Ripley, “Handbook of Birds of India and Pakistan”, Oxford University Press, Oxford, 1983.
  [13]. S. Asokan, “Food and feeding habits of the Small Green Bee-eater Merops orientalis in Mayiladuthurai”. Journal of Ecobiology, Vol. 10, Issue. 3, pp. 199-204, 1998.
  [14]. S. Asokan, Ali, A.M.S., and Manikannan, R. “Breeding biology of the small bee-eaters, Merops orientalis in Nagapattinam district, Tamil Nadu, India”. Journal of Threatened Taxa, Vol. 2, Issue.4 pp. 797-804, 2010.
  [15]. S. Mishra, “Nesting and parental care in Cinnyris jugularis”. International Journal of Engineering Applied Sciences and Technology, Vol. 4, Issue 4, pp. 119-122, 2019.
  [16]. S. Sridhar, and K.P. Karanth, “Helpers in cooperatively breeding small green bee-eaters (Merops orientalis)”, Current Science Vol.65, Issue.6, pp. 489-490, 1993.
  

  Citation
  Swetaleena Mishra, "The Ecology of Merops Orientalis," International Journal of Scientific Research in Biological Sciences, Vol.6, Issue.5, pp.12-14, 2019

• Open Access   Article

  Evaluation of Phytochemicals and In Vitro Anticancer Activity of Eupatorium Triplinerve

  Suja G., Vinoth Kumar T., Suganya P.

  Research Paper | Journal-Paper (IJSRBS)

  Vol.6 , Issue.5 , pp.15-18, Oct-2019

  CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6i5.1518

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  The present study attempts to evaluate the phytochemical activity and in vitro anticancer activity of Eupatorium triplinerve methanol extract against lung cancer cell line A-549 and normal cell line Vero. The compound was analyzed for the presence of various phytoconstituents and screened for its cytotoxicity at five different concentrations by MTT assay. The phytochemical profile exhibited the presence of alkaloids, phenolics, flavonoids and steroids. The study documented that the percentage of cell viability has been reduced with the increased concentration, as evidenced by cell death. Eupatorium triplinerve extract shows potential cytotoxic activity with CTC50 of 450 µg/ml and 110 µg/ml against A-549 and Vero cell line respectively. The result obtained was comparably potent, the crude extract showed moderate activity against cancer cells and it was less toxic to the normal cells. In future, research need to be explored in the purification of this extract and identification of lead compound which could be used as a therapeutic tool against lung cancer

  Key-Words / Index Term
  Anticancer, In vitro, Plant extract, Eupatorium triplinerve, Cytotoxicity, MTT assay

  References
  [1]. S.K. Pal and B. Mittal B, “Fight against cancer in countries with limited resources: the post-genomic era scenario”, Asian Pacific Journal of Cancer Prevention, Vol. 5, Issue 3, pp. 328-33, 2004.
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  [8]. W. Strober, ‘Tryphan blue exclusion test of cell viability”, Current Protocol of Immunology, John Wiley & Sons publishers, U.K, pp. A3.B.1- A3.B.2D, 1997
  [9]. A. Scudiero, R.H. Shoemaker, K. D. Paul, “Evaluation of soluble Tetrazolium Formazan assay for cell growth and drug sensitivity in cultures using human and other tumor cell lines”, Cancer Research. Vol. 48, pp. 4827- 4833, 1998.
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  [12]. M.S. Uma Maheswari, R. Rajendran, “Comparative Evaluation of Qualitative and Quantitative Phytochemical Analysis of South Indian Bioactive Medicinal Plant Tribulus terrestris”, International Journal of Scientific Research in Biological Sciences, Vol.5, Issue.6, pp.144-149, 2018.
  [13]. K. F. Sigaroodi, M. Jeddi-Tehrani, M. Ahvazi M, “Cytotoxicity evaluation of Taverniera spartea on human cancer cell lines”, Journal of Medicinal Plants. Vol.2. pp.114-128, 2014.
  [14]. J. Guna Priya, K. Geetha Lakshmi, Niharika Sharma, D. Rajani, “A Comparative Study of Antioxidants and Antimicrobial activity in Vegetables, Leafy Vegetables and Spices”, International Journal of Scientific Research in Biological Sciences, Vol.06, Issue.01, pp.80-85, 2019.
  

  Citation
  Suja G., Vinoth Kumar T., Suganya P., "Evaluation of Phytochemicals and In Vitro Anticancer Activity of Eupatorium Triplinerve," International Journal of Scientific Research in Biological Sciences, Vol.6, Issue.5, pp.15-18, 2019

• Open Access   Article

  Diversity and Distribution of Phytoplankton at selected sites of Jamnagar coast, Gujarat, India

  Roshan Bhagat, Rajal Patel, Harshad Salvi, Kamboj R.D.

  Research Paper | Journal-Paper (IJSRBS)

  Vol.6 , Issue.5 , pp.19-25, Oct-2019

  CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6i5.1925

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  Abstract
  Phytoplanktons are the primary producer of marine ecosystems and contribute towards the primary productivity of aquatic environment. The present study was carried out at three selected sites of Gulf of Kachchh (GoK) viz; Pirotan, Sachana and Roziport. During the study, a total of 106 species of phytoplankton were recorded, of which the highest species diversity was found at Rozi followed by Pirotan and Sachana. The maximum number of species diversity was contributed by the class Bacillariophyceae with 81.5 percentage , 79 percentage and 83 percentage at Pirotan, Rozi and Sachana, respectively. Rozi port reported the highest number of harmful phytoplankton i.e. some members of Bacillariophyceae and Dinophyceae. The highest number of species was recorded at Rozi port (75 sp.) followed by Pirotan (54 sp.) and Sachana (46 sp.). The varied environmental conditions prevailing at three study sites may cause variation in species composition at these sites

  Key-Words / Index Term
  Coral reef, Island, Mangrove, Phytoplankton, Port, Sewage, ship breaking yard

  References
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  [5]. Vethamony P. and M.T. Babu, “Physical process in Gulf of Kachchh: A Review Indian”, J. Geo-Mar. Sci. 39(4), 497-503, 2010.
  [6]. Sen Gupta, R. and G. Deshmukhe, “Coastal and maritime environments of Gujarat: Ecology and Economics.”, Gujarat Ecological Society, pp. 150, 2000.
  [7]. Singh H. S., C.N. Pandey, P. Yennawar, R.J. Asari, B.H. Patel, K. Tatu, and B.R. Raval, “ The marine national park and sanctuary in the Gulf of Kachchh–a comprehensive study on biodiversity and management issues.” GEER Foundation, Gandhinagar, 2004.
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  [10]. Rajasekar K. Thillai, M. Rajkumar, Jun Sun, Prabu V. Ashok, P. Perumal, ”Seasonal variations of phytoplankton diversity in the Coleroon coastal waters, southeast coast of India”,Acta Oceanol. Sin., , Vol. 29, No. 5, P. 97-108, 2010.
  [11]. Dayala, V.T., P.M. Salas, C.H. Sujatha, "Spatial and seasonal variations of phytoplankton species and their relationship to physicochemical variables in the Cochin estuarine waters, Southwest Coast of India" Indian Journal of Geo-Marine Sciences, Vol. 43 (6), pp. 937-947, 2013.
  [12]. Subrahmanyan, R. and A.H. Viswanatha Sarma, “Studies on the phytoplankton of the west coast of India. Part III. Seasonal variation of the phytoplankters and environmental factors.”, Indian J. Fish., 7 (2): 307-336, 1960.
  [13]. Fehling,J., K. Davidson, C.J. Bolch, and S.S. Bates, “Growth and domoic acid production by Pseudo‐nitzschiaseriata (Bacillariophyceae) under phosphate and silicate limitation.” Journal of Phycology, 40 (4), 674-683, 2004.
  [14]. Tsutsumi, H., “Critical events in the Ariake Bay ecosystem: clam population collapse, red tides, and hypoxic bottom water”, Plankton and Benthos Research, 1(1), 3-25.
  [15]. Al-Kandari, M., F.Al-Yamani,and K. Al-Rifaie, “Marine phytoplankton atlas of Kuwait’s waters. Kuwait Institute for Scientific Research (Publisher),” 2009.
  [16]. Kedong, Yin Monsoonal, “Influence on seasonal variations in nutrients and phytoplankton biomass in coastal waters of Hong Kong in the vicinity of the Pearl River estuary.” Mar EcolProgSer 245: 111–122 2002.
  [17]. Panda S.S., N.K.Dhal, andC.R. Panda, “Phytoplankton diversity in response to abiotic factors along Orissa coast, Bay of Bengal. Int J Environ Sci, 2, 3-8 2012.
  [18]. Pandiyarajan, R.S., P.S.Shenai-Tirodkar, M. AyajuddinandZ.A. Ansari, “Distribution, abundance and diversity of Phytoplankton in the inshore waters of Nizampatnam, South East coast of India”, India Journal of Geo –Marine Science, Vol. 43 (3), pp. 348 – 356 March 2014.
  [19]. Shruthi M.S., and M. Rajashekhar, “Ecological observations on the phytoplankton of Netravathi-Gurupura estuary, South west coast of India.” J. Mar. Biol. Ass. India, 55, 1-7, 2014.
  

  Citation
  Roshan Bhagat, Rajal Patel, Harshad Salvi, Kamboj R.D., "Diversity and Distribution of Phytoplankton at selected sites of Jamnagar coast, Gujarat, India," International Journal of Scientific Research in Biological Sciences, Vol.6, Issue.5, pp.19-25, 2019

• Open Access   Article

  Induction of ovarian toxicity in the freshwater fish, Pseudetroplus maculatus (Bloch, 1795) after sublethal exposure of dibutyl phthalate

  K. Sajla, K. P. Raibeemol, K. C. Chitra

  Research Paper | Journal-Paper (IJSRBS)

  Vol.6 , Issue.5 , pp.26-38, Oct-2019

  CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6i5.2638

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  Abstract
  Dibutyl phthalate, a plasticizer, is widely used as a softener in several commercial industrial products. The study investigated the sublethal effects of dibutyl phthalate on ovary of the freshwater fish, Pseudetroplus maculatus. Dibutyl phthalate exposed at 0.2 mg/ L concentration for 24, 72 and 96 h showed behavioural modifications in the fish. The body weight showed significant reduction after 72 h of toxicant exposure with concomitant increase in mucous deposition, which indicated whole-organism response against the toxicant. The weight of ovary and gonadosomatic index decreased significantly in all treatment groups thereby suggested the stress exhibited on female reproductive organ. Dibutyl phthalate prevented normal activities of steroidogenic enzymes as 3β- and 17β-hydroxysteroid dehydrogenase in ovary of the fish. The alterations in the level of vitellogenin measured through indirect endpoints such as alkali-labile phosphoprotein and total protein in ovary and blood plasma indicated the estrogenic effects of the toxicant. Dibutyl phthalate disrupted hypothalamo-pituitary-gonadal axis as demonstrated by the increase in the levels of luteinizing hormone and testosterone with reduction in serum estradiol without remarkable changes in the level of follicle stimulating hormone. In the ovary, number of vitellogenic oocyte increased whereas the population of pre-vitellogenic oocyte declined without any changes in the number of post-vitellogenic oocyte. Histological evaluation revealed severe morphological damages associated to apoptosis such as empty follicle and atretic oocytes, membrane blebbing, nuclear condensation, vacuolization and broken theca granulosa membrane. The present findings suggested that sublethal exposure of DBP induced ovarian toxicity in the freshwater fish, Pseudetroplus maculatus

  Key-Words / Index Term
  Dibutyl phthalate, Steroidogenic enzymes, Hormones, Sublethal toxicity, Histology, Pseudetroplus maculatus

  References
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  Citation
  K. Sajla, K. P. Raibeemol, K. C. Chitra, "Induction of ovarian toxicity in the freshwater fish, Pseudetroplus maculatus (Bloch, 1795) after sublethal exposure of dibutyl phthalate," International Journal of Scientific Research in Biological Sciences, Vol.6, Issue.5, pp.26-38, 2019

• Open Access   Article

  Screening of Endophytes from Traditionally Used Medicinal Plants of Manipur for Their Antimicrobial Activity: An Impact towards Future Drug Discovery

  Ng Ngashangva, Indira Devi S, Kalita M. C.

  Research Paper | Journal-Paper (IJSRBS)

  Vol.6 , Issue.5 , pp.39-47, Oct-2019

  CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6i5.3947

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  Abstract
  Antimicrobial resistance has been developing fast and continuing to challenge the world medical sector. New strategies are needed to address this challenge that is incurring huge loss on human life. Medicinal plants from the untapped rainforest of the North-East India has a huge potential to find microbes that produce novel biomolecules. Bioactive endophytes were isolated from traditionally used medicinal plants. Paenibacillus spp. from Piscidia spp. in a fermentation broth has shown strong and broad-spectrum of activity at the range of 1600 AU/ml. Unexplored region has huge prospect to find novel biomolecules which can be harvested in scientific, economical and environmentally friendly. Moreover, it has shown that the traditional medicinal plant harboured bioactive. Moreover, it will provide insights into its metabolic potential and ecological role of this species

  Key-Words / Index Term
  Endophyte, antimicrobial, supernatant, resistance, medicinal plants

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  Citation
  Ng Ngashangva, Indira Devi S, Kalita M. C., "Screening of Endophytes from Traditionally Used Medicinal Plants of Manipur for Their Antimicrobial Activity: An Impact towards Future Drug Discovery," International Journal of Scientific Research in Biological Sciences, Vol.6, Issue.5, pp.39-47, 2019

• Open Access   Article

  An Investigation of Soil micro faunal diversity in chemical effluents contaminated soil versus normal soil at A. B. N. Seal College Campus, Cooch Behar, West Bengal

  Debojyoti Dutta

  Research Paper | Journal-Paper (IJSRBS)

  Vol.6 , Issue.5 , pp.48-52, Oct-2019

  CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6i5.4852

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  Abstract
  A B N Seal College, Cooch Behar is a 131 years old premier general Degree College of West Bengal. Since it is in Government premises, therefore there is not much alteration of soil type over here. Chemistry Department has its 47 years old glory. So, it is obvious that chemical effluents which are going directly into soil will be contaminated since the time of establishment of this Department. In this paper an attempts are undertaken to make a comparative analysis of Soil micro faunal diversity in chemical effluents contaminated soil versus normal soil at A. B. N. Seal College Campus, Cooch Behar, West Bengal. Here not only surface soil is considered but also different depth of the soil is also giving much importance as a parameter. All microscopical analyses are being done under 400X magnification with photographic attachment. Micro arthropod, mites and ants diversity in terms of number and species are varied from control site to experimental sites. Large number degenerated nematode eggs are found in upper surface of the experimental sites. Lesion nematodes were found in all the depths of the investigated soil. Depth below 6 to 12 inches, presence cysts of nematodes indicates adverse environmental condition for their sustainability

  Key-Words / Index Term
  Nematodes, Mites, Cyst, Soil, Micro arthropod

  References
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  Citation
  Debojyoti Dutta, "An Investigation of Soil micro faunal diversity in chemical effluents contaminated soil versus normal soil at A. B. N. Seal College Campus, Cooch Behar, West Bengal," International Journal of Scientific Research in Biological Sciences, Vol.6, Issue.5, pp.48-52, 2019

• Open Access   Article

  Study on soil analysis in ponds in the Sabang block areas, West Bengal, India

  Uttam Kumar Maji, Ranajit Kumar Khalua, Kartik Maiti

  Research Paper | Journal-Paper (IJSRBS)

  Vol.6 , Issue.5 , pp.53-63, Oct-2019

  CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6i5.5363

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  Abstract
  Bacteria live in fresh water, soil, air and sea water. Bacteria have different nature in different zone. Thus periphyton bacteria has different characteristic in different zone. But I discussed in my topic soil analysis in fresh water ponds in Sabang block areas. I have done the experiment about one year in Sabang block areas. Soil collected by the bucket. This collected soil is collected from five ponds (Ghat pond, Dhara pond, Majari pond, Nandan pond & Masanta pond) in Sabang block areas. There were two types of pond present Control pond and Experimental pond. In this pond was added the semidried cow dank. In this experiment we are followed the some methods. The pond bottom soil was analyzed for nitrogen, available phosphorus and available potassium following the standard methods of AOAC (1980). In all the experimental ponds before treatment however, after treatment the nitrogen level increased considerable. Phosphorus plays significant role in enhancing the productivity of pond. Potassium is not limiting factor and is not necessary to increase production

  Key-Words / Index Term
  Soil analysis, nitrogen, phosphorus, potassium, ponds

  References
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  Citation
  Uttam Kumar Maji, Ranajit Kumar Khalua, Kartik Maiti, "Study on soil analysis in ponds in the Sabang block areas, West Bengal, India," International Journal of Scientific Research in Biological Sciences, Vol.6, Issue.5, pp.53-63, 2019

• Open Access   Article

  Acaulospora paulinae, a new record of arbuscular mycorrhizal fungal species from Telangana state, India

  K. Hari Prasad, A. Hindumathi, B. Bhadraiah

  Research Paper | Journal-Paper (IJSRBS)

  Vol.6 , Issue.5 , pp.64-66, Oct-2019

  CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6i5.6466

  Abstract

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  Citation

  Abstract
  Acaulospora paulinae was isolated from the rhizosphere soils of Setaria italica (Poaceae), Emilia sonchifolia and Vernonia cinerea (Asteraceae) from Nagarkurnool and Wanaparthy Districts, Telangana, India. The spores are globose to subglobose, 60-95µm in diameter. Sometimes ovoid 60-70 X 80-95 µm. Hyaline to sub hyaline or pale yellow. The spore wall composed of three layers. The surface of the middle layer is ornamented with evenly distributed pits 2.0-2.5 X 3.0-3.5 µm. A. paulinae spores were found in sandy loam soils with decreasing spore numbers. A. paulinae is a characteristic member of the arbuscular mycorrhizal fungal communities in soils with pH 5.3-8.5 in sandy loam soils of Nagarkurnool and Wanaparthy Districts, Telangana, India

  Key-Words / Index Term
  Acaulospora paulinae, Arbuscular mycorrhizal fungi, Glomeromycota, Rhizosphere, Acaulosporaceae, Spore morphology, Taxonomy

  References
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  Citation
  K. Hari Prasad, A. Hindumathi, B. Bhadraiah, "Acaulospora paulinae, a new record of arbuscular mycorrhizal fungal species from Telangana state, India," International Journal of Scientific Research in Biological Sciences, Vol.6, Issue.5, pp.64-66, 2019