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• Open Access   Article

  Analysis of In-vitro antioxidant properties of Green synthesized Ni/reduced graphene oxide composite

  Gamage HMGMP, Madushani WSAT, Pahalagedara MN

  Research Paper | Journal-Paper (IJSRCS)

  Vol.11 , Issue.4 , pp.1-10, Aug-2024

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  Nickel nanoparticles (NiNPs) possess numerous health benefits that are useful in biomedical applications. Synthesis of NPs via green approaches has become prominent nowadays due to their cost effective, and eco-friendly manner. In the current study, Ni NPs were synthesized via a green approach. As a support to disperse and stabilize NPs, reduced graphene oxide (rGO) was used while preventing agglomeration. Then the in-vitro antioxidant activity of synthesized composites was examined. As the reducing and capping agent Azadirachta indica (Neem) extract was used in the synthesis process. All Ni/rGO composites synthesized possessed antioxidant activity in concentration dependent manner and activity was always greater than rGO. The highest activity was shown by Neem/NiNPs where inhibition was 78% for a 250 ug/ml sample. Energy Dispersive X-Ray (EDX) analysis, shows signals of Ni along with oxygen and carbon confirming the incorporation of NiNPs into composite. Among composites, the highest activity was shown by Ni/rGO (RT) where inhibition was 63% and EC50 was 35ug/ml. This contained only 17 weight% Ni. These results confirm that after the formation of the composite, even a lower amount of NiNPs show considerably high activity due to the formation of mono-dispersed layer of NPs and phytochemicals attached on rGO. Antioxidant activity has greatly enhanced due to the synergetic effects between NiNPs and rGO sheets. These results suggest that Neem extract can be extensively used in green production of Ni/rGO composite which is an excellent candidate for safe biomedical, pharmaceutical as well as other industrial applications.

  Key-Words / Index Term
  Nanoparticles, Azadirachta indica, Antioxidant, Reduced Graphene oxide, Phytochemicals, Nickel

  References
  [1] N. A. N. Mohamad, N. A. Arham, J. Jai, and A. Hadi, “Plant ex-tract as reducing agent in synthesis of metallic nanoparticles: A review,” in Advanced Materials Research, pp. 350–355, 2014.
  [2] M. A. Al-Hubaishi, S. Gaikwad, and A. S. Rajbhoj, “Enhanced antimicrobial activity of Zinc oxide nanoparticles with controled particle size by current density,” International Journal of Scientific Research in Chemical Sciences, Vol.6, Issue.1, pp.1–9, 2019.
  [3] P. Roy, B. Das, A. Mohanty, and S. Mohapatra, “Green synthesis of silver nanoparticles using azadirachta indica leaf extract and its antimicrobial study,” Applied Nanoscience (Switzerland), Vol.7, Issue.8, pp.843–850,2017.
  [4] Y. Bao, J. He, K. Song, J. Guo, X. Zhou, and S. Liu, “Plant-Extract-Mediated Synthesis of Metal Nanoparticles,” 2021, Hindawi Limited. 2021.
  [5] N. Sampathkumar and A. Swetharnayam, “Pharmacological Potential of TiO2 Nanoparticles synthesized by Chemical and Green method-A Comparative study,” International Journal of Scientific Research in Chemical Sciences, Vol.11, Issue.2, 2024.
  [6] M. A. Alzohairy, “Therapeutics role of azadirachta indica (Neem) and their active constituents in diseases prevention and treat-ment,” Hindawi Limited. 2016.
  [7] S. Iravani, “Green synthesis of metal nanoparticles using plants,” Green Chemistry, Vol.13, Issue.10, pp.2638–2650, 2011.
  [8] T. K. Ghorai, “Graphene oxide-based nanocomposites and biomedical applications,” in Functional Polysaccharides for Biomedical Applications, Elsevier, pp.305–328, 2019.
  [9] A. W. Anwar, W. Ullah, R. Ahmad, A. Majeed, N. Iqbal, and A. Khan, “Simple and inexpensive synthesis of rGO-(Ag, Ni) nano-composites via green methods,” Materials Technology, Vol.30, Issue.3, pp. 155–160, 2015.
  [10] N. D. Jaji, H. L. Lee, M. H. Hussin, H. M. Akil, M. R. Zakaria, and M. B. H. Othman, “Advanced nickel nanoparticles technology: From synthesis to applications,”, De Gruyter Open Ltd. 2020.
  [11] J. Singh, T. Dutta, K. H. Kim, M. Rawat, P. Samddar, and P. Ku-mar, “‘Green’ synthesis of metals and their oxide nanoparticles: Applications for environmental remediation”, BioMed Central Ltd. 2018.
  [12] H. R. El-Seedi et al., “Metal nanoparticles fabricated by green chemistry using natural extracts: Biosynthesis, mechanisms, and applications,”, Royal Society of Chemistry. 2019
  [13] S. Sudhasree, A. Shakila Banu, P. Brindha, and G. A. Kurian, “Synthesis of nickel nanoparticles by chemical and green route and their comparison in respect to biological effect and toxicity,” Toxicol Environ Chem, Vol.96, Issue.5, pp.743–754, 2014.
  [14] P. Bharat, R. Sagar, R. Sulav, and P. Ankit, “Investigations of antioxidant and antibacterial activity of leaf extracts of Aza-dirachta indica,” Afr J Biotechnol, Vol.14, Issue.46, pp. 3159–3163, 2015.
  [15] V. Helan et al., “Neem leaves mediated preparation of NiO nano-particles and its magnetization, coercivity and antibacterial analy-sis,” Results in Physics, Vol.6, pp.712–718, 2016.
  [16] B. A. Abbasi, J. Iqbal, T. Mahmood, R. Ahmad, S. Kanwal, and S. Afridi, “Plant-mediated synthesis of nickel oxide nanoparticles (NiO) via Geranium wallichianum: Characterization and different biological applications,” Mater Res Express, Vol.6, Issue.8, 2019.
  [17] Y. Huang, C. Zhu, R. Xie, and M. Ni, “Green synthesis of nickel nanoparticles using Fumaria officinalis as a novel chemotherapeutic drug for the treatment of ovarian cancer,” J Exp Nanosci, Vol.16, Issue.1, pp.369–382, 2021.
  [18] A. A. Olorunkosebi et al., “Optimization of graphene oxide through various Hummers’ methods and comparative reduction using green approach,” Diam Relat Mater, Vol.117, 2021.
  [19] Guibin Lou et al., “Study on the antibacterial and anti-corrosion properties of Ni-GO/Ni-rGO composite coating on manganese steel,” China, 2021.
  [20] I. G. Munteanu and C. Apetrei, “Analytical methods used in determining antioxidant activity: A review,”, MDPI AG. 2021.
  [21] D. T. Handago, E. A. Zereffa, and B. A. Gonfa, “Effects of Aza-dirachta Indica Leaf Extract, Capping Agents, on the Syn-thesis of Pure and Cu Doped ZnO-Nanoparticles: A Green Approach and Microbial Activity,” Open Chem, Vol.17, Issue.1, pp.246–253, 2019.
  [22] K. Girish, “Neem (Azadirachta indica A. Juss) as a source for green synthesis of nanoparticles”, Innovare Academics Sciences Pvt. Ltd. 2018
  [23] Y. Tian, Y. Liu, F. Pang, F. Wang, and X. Zhang, “Green synthesis of nanostructed Ni-reduced graphene oxide hybrids and their application for catalytic reduction of 4-nitrophenol,” Colloids Surf A Physicochem Eng Asp, Vol.464, pp.96–103, 2015.
  [24] P. Das, S. Ghosh, and M. Baskey, “Heterogeneous catalytic reduction of 4-nitroaniline by RGO-Ni nanocomposite for water resource management,” Journal of Materials Science: Materials in Electronics, Vol.30, Issue.22, pp.19731–19737, 2019.
  [25] A. Al-Nafiey, M. H. K. Al-Mamoori, S. M. Alshrefi, A. K. Shakir, and R. T. Ahmed, “One step to synthesis (rGO/Ni NPs) nanocom-posite and using to adsorption dyes from aqueous solution,” 2019.
  [26] N. N. Malinga and A. L. L. Jarvis, “Synthesis, characterization and magnetic properties of Ni, Co and FeCo nanoparticles on reduced graphene oxide for removal of Cr (VI),” J Nanostructure Chem, Vol.10, Issue.1, pp.55–68, 2020.
  [27] D. A. Kriz, J. He, M. Pahalagedara, and S. L. Suib, “Response to Comments on the application of the Scherrer equation in “Copper aluminum mixed oxide (CuAl MO) catalyst: A green approach for the one-pot synthesis of imines under solvent-free conditions”, Appl. Catal. B: Environ, Vol.188, pp.227–234, 2016.
  [28] S. B. Kedare and R. P. Singh, “Genesis and development of DPPH method of antioxidant assay,” 2011.

  Citation
  Gamage HMGMP, Madushani WSAT, Pahalagedara MN, "Analysis of In-vitro antioxidant properties of Green synthesized Ni/reduced graphene oxide composite," International Journal of Scientific Research in Chemical Sciences, Vol.11, Issue.4, pp.1-10, 2024

• Open Access   Article

  Age Estimation of Concealed and Unconcealed Blood Stains on Different Surfaces – A Chemometric Approach

  Deepthi Nagesh, B. Nagarajamurthy, Bhanuprakash R.

  Research Paper | Journal-Paper (IJSRCS)

  Vol.11 , Issue.4 , pp.11-22, Aug-2024

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  Blood stains are commonly encountered at crime scenes, either in visible or hidden forms. Establishing the age of these blood stains is critical for effective crime scene investigation. The current study aimed to develop regression models for accurately estimating the age of concealed blood stains on diverse surfaces. Additionally, the study investigated the effects of luminol and concealment on age estimation. The study utilized ATR-FTIR spectroscopy in combination with Chemometric methods, including PCA (Principal Component Analysis) and OPLSR (Orthogonal Signal Correction Partial Least Square Regression Analysis), to analyze blood stains concealed by paint and treated with luminol on three commonly encountered substrates: cement, metal, and wood. The spectral data were analyzed to develop regression models to estimate the age of blood stains for eleven days. The regression models for concealed blood stains exhibited notable differences compared to those for unconcealed blood stains. The predictive Root Mean Square Error (RMSE) values ranged from 0.87 to 1.82, and R-squared values ranged from 0.76 to 0.94. The model for blood stains on cement surfaces was the most accurate in concealed form, while the model for blood stains on metal surfaces was the best fit in unconcealed form. Furthermore, the model for concealed blood stains on wooden surfaces demonstrated the highest prediction error. The results indicated that the presence of luminol and the composition of the substrates on which blood stains were identified significantly influenced the detection of spectral changes associated with age-related alterations observed in blood stains.

  Key-Words / Index Term
  Blood stains, ATR-FTIR Spectroscopy, Chemometrics, PCA, PLSR, Concealed blood stains, Luminol, Nature of Substrates, Cement, Wood, Metal

  References
  [1] Richard Li, Forensic Biology. New York: Taylor and Francis Group CRC Press, 2011.
  [2] R. H. Bremmer, A. Nadort, T. G. van Leeuwen, M. J. C. van Gemert, and M. C. G. Aalders, “Age estimation of blood stains by hemoglobin derivative determination using reflectance spectroscopy,” Forensic Sci Int, vol. 206, no. 1–3, pp. 166–171, Mar. 2011, doi: 10.1016/j.forsciint.2010.07.034.
  [3] D. Nagesh and B. Nagarajamurthy, “Estimation of Time-Since-Deposition of bloodstains on different surfaces using ATR-FTIR Spectroscopy and Chemometrics,” Forensic Sci Med Pathol, Jul. 2024, doi: 10.1007/s12024-024-00849-w.
  [4] F. Barni, S. W. Lewis, A. Berti, G. M. Miskelly, and G. Lago, “Forensic application of the luminol reaction as a presumptive test for latent blood detection,” May 15, 2007, Elsevier. doi: 10.1016/j.talanta.2006.12.045.
  [5] A. Castelló, M. Alvarez, and F. Verdú, “Accuracy, reliability, and safety of luminol in bloodstain investigation,” Journal of the Canadian Society of Forensic Science, vol. 35, no. 3, pp. 113–121, 2002, doi: 10.1080/00085030.2002.10757540.
  [6] S. Strasser et al., “Age determination of blood spots in forensic medicine by force spectroscopy,” Forensic Sci Int, vol. 170, no. 1, pp. 8–14, Jul. 2007, doi: 10.1016/j.forsciint.2006.08.023.
  [7] A. R. Weber and I. K. Lednev, “Crime clock – Analytical studies for approximating time since deposition of bloodstains,” Forensic Chemistry, vol. 19, Jun. 2020, doi: 10.1016/j.forc.2020.100248.
  [8] A. Kumar Gautam, A. Verma, and A. Vyas, “An Overview of Age Estimation in Forensic Science: Based on Techniques and Methods,” International Journal of Scientific Research in Multidisciplinary Studies, vol. 9, no. 10, pp. 1–11, 2023, doi: 10.26438/ijsrms/v9i10.111.
  [9] Y. Fujita, K. Tsuchiya, S. Abe, Y. Takiguchi, S. I. Kubo, and H. Sakurai, “Estimation of the age of human bloodstains by electron paramagnetic resonance spectroscopy: Long-term controlled experiment on the effects of environmental factors,” Forensic Sci Int, vol. 152, no. 1, pp. 39–43, Aug. 2005, doi: 10.1016/j.forsciint.2005.02.029.
  [10] A. V. Pandya and R. T. Nair, “Spectroscopic Analysis of Conducting Polymeric Material ,” International Journal of Scientific Research in Chem International Journal of Scientific Research in Chemical Sciences, vol. 1, no. 1, 2014.
  [11] S. E. Glassford, B. Byrne, and S. G. Kazarian, “Recent applications of ATR FTIR spectroscopy and imaging to proteins,” 2013, Elsevier B.V. doi: 10.1016/j.bbapap.2013.07.015.
  [12] A. Takamura, K. Watanabe, T. Akutsu, and T. Ozawa, “Soft and Robust Identification of Body Fluid Using Fourier Transform Infrared Spectroscopy and Chemometric Strategies for Forensic Analysis,” Sci Rep, 2018, doi: 10.1038/S41598-018-26873-9.
  [13] R. Kumar and V. Sharma, “Chemometrics in forensic science,” Aug. 01, 2018, Elsevier B.V. doi: 10.1016/j.trac.2018.05.010.
  [14] S. Sharma, R. Chophi, J. K. Jossan, and R. Singh, “Detection of bloodstains using attenuated total reflectance-Fourier transform infrared spectroscopy supported with PCA and PCA–LDA,” Med Sci Law, vol. 61, no. 4, pp. 292–301, Oct. 2021, doi: 10.1177/00258024211010926.
  [15] G. Edelman, T. G. van Leeuwen, and M. C. G. Aalders, “Hyperspectral imaging for the age estimation of blood stains at the crime scene,” Forensic Sci Int, vol. 223, no. 1–3, pp. 72–77, Nov. 2012, doi: 10.1016/j.forsciint.2012.08.003.
  [16] T. Das, A. Harshey, K. Nigam, V. K. Yadav, and A. Srivastava, “Analytical approaches for bloodstain aging by vibrational spectroscopy: Current trends and future perspectives,” Microchemical Journal, 2020, doi: 10.1016/J.MICROC.2020.105278.
  [17] R. H. Bremmer, K. G. de Bruin, M. J. C. van Gemert, T. G. van Leeuwen, and M. C. G. Aalders, “Forensic quest for age determination of bloodstains.,” Forensic Sci Int, 2012, doi: 10.1016/J.FORSCIINT.2011.07.027.
  [18] G. Zadora and A. Men?yk, “In the pursuit of the holy grail of forensic science – Spectroscopic studies on the estimation of time since deposition of bloodstains,” Trends in Analytical Chemistry, 2018, doi: 10.1016/J.TRAC.2018.04.009.
  [19] A. Marrone and J. Ballantyne, “Changes in dry state hemoglobin over time do not increase the potential for oxidative DNA damage in dried blood,” PLoS One, vol. 4, no. 4, Apr. 2009, doi: 10.1371/journal.pone.0005110.
  [20] E. K. Hanson and J. Ballantyne, “A blue spectral shift of the hemoglobin soret band correlates with the age (time since deposition) of dried bloodstains,” PLoS One, vol. 5, no. 9, pp. 1–11, 2010, doi: 10.1371/journal.pone.0012830.
  [21] A. R. Weber and I. K. Lednev, “Crime clock – Analytical studies for approximating time since deposition of bloodstains,” Forensic Chemistry, 2020, doi: 10.1016/J.FORC.2020.100248.
  [22] K. C. Doty, G. McLaughlin, and I. K. Lednev, “A Raman ‘spectroscopic clock’ for bloodstain age determination: the first week after deposition,” Anal Bioanal Chem, 2016, doi: 10.1007/S00216-016-9486-Z.
  [23] E. Mistek, L. Halámková, K. C. Doty, C. K. Muro, and I. K. Lednev, “Race Differentiation by Raman Spectroscopy of a Bloodstain for Forensic Purposes,” Anal Chem, vol. 88, no. 15, pp. 7453–7456, Aug. 2016, doi: 10.1021/acs.analchem.6b01173.
  [24] E. Mistek and I. K. Lednev, “Identification of species’ blood by attenuated total reflection (ATR) Fourier transform infrared (FT-IR) spectroscopy,” Anal Bioanal Chem, vol. 407, no. 24, pp. 7435–7442, Sep. 2015, doi: 10.1007/s00216-015-8909-6.
  [25] H. Sun et al., “Non-invasive prediction of bloodstain age using the principal component and a back propagation artificial neural network,” Laser Phys Lett, vol. 14, no. 9, Sep. 2017, doi: 10.1088/1612-202X/aa7c48.
  [26] H. Lin, Y. Zhang, Q. Wang, B. Li, P. Huang, and Z. Wang, “Estimation of the age of human bloodstains under the simulated indoor and outdoor crime scene conditions by ATR-FTIR spectroscopy,” Sci Rep, vol. 7, no. 1, Dec. 2017, doi: 10.1038/s41598-017-13725-1.
  [27] R. Kumar, K. Sharma, and V. Sharma, “Bloodstain age estimation through infrared spectroscopy and Chemometric models.,” Science & Justice, 2020, doi: 10.1016/J.SCIJUS.2020.07.004.
  [28] S. H. James, P. E. Kish, and T. P. Sutton, Principles of Bloodstain Pattern Analysis. CRC Press, 2005. doi: 10.1201/9781420039467.
  [29] T. L. Laberj and R. P. Epstein2, “ARTICLES SUBSTRATE EFFECTS ON THE CLOTTING TIME OF HUMAN BLOOD,” 2001.
  [30] Andrasko J, “The estimation of age of bloodstains by HPLC analysis,” J Forensic Sci, pp. 601–607, 1997.
  [31] E. Botoniic-Sehic, C. Brown, M. Lamontaane, and M. Tsaparikos, “Forensic Application of Near-Infrared Spectroscopy: Aging of Bloodstains,” Spectroscopy (Santa Monica), vol. 24, Jun. 2009.
  [32] P. Lemler, W. R. Premasiri, A. DelMonaco, and L. D. Ziegler, “NIR Raman spectra of whole human blood: effects of laser-induced and in vitro hemoglobin denaturation,” Anal Bioanal Chem, vol. 406, no. 1, pp. 193–200, Jan. 2014, doi: 10.1007/s00216-013-7427-7.
  [33] R. Kumar, K. Sharma, and V. Sharma, “Bloodstain age estimation through infrared spectroscopy and Chemometric models,” Science and Justice, vol. 60, no. 6, pp. 538–546, Nov. 2020, doi: 10.1016/j.scijus.2020.07.004.
  [34] S. H. James, P. E. Kish, and T. P. Sutton, Principles of Bloodstain Pattern Analysis. CRC Press, 2005. doi: 10.1201/9781420039467.
  [35] L. T. , & H. D. G. Lytle, “Chemiluminescence in the visualization of forensic bloodstains. ,” J Forensic Sci, vol. 23(3), pp. 550–562, 1978.
  [36] N. F. N. Hassan, D. D. Sandran, M. Mohamad, Y. Zakaria, and N. Z. M. Muslim, “Estimation of the age of bloodstains on soil matrices by ATR-FTIR spectroscopy and chemometrics,” International Journal of Innovative Technology and Exploring Engineering, vol. 9, no. 1, pp. 4750–4755, Nov. 2019, doi: 10.35940/ijitee.A4454.119119.
  [37] A. M. , H. K. A. , & K. G. L. Gross, “The effect of luminol on presumptive tests and DNA analysis using the polymerase chain reaction. ,” J Forensic Sci, vol. 44(4), pp. 837–840, 1999.
  [38] D. Patzelt, “History of forensic serology and molecular genetics in the sphere of activity of the German Society for Forensic Medicine,” Forensic Sci Int, vol. 144, no. 2–3, pp. 185–191, Sep. 2004, doi: 10.1016/j.forsciint.2004.04.053.
  [39] A. , & M. S. Della Manna, “A novel approach to obtaining reliable PCR results from luminol treated bloodstains.,” J Forensic Sci, vol. 45(4), pp. 886–890, 2000.
  [40] B. Li, P. Beveridge, W. T. O’Hare, and M. Islam, “The age estimation of blood stains up to 30 days old using visible wavelength hyperspectral image analysis and linear discriminant analysis,” Science and Justice, vol. 53, no. 3, pp. 270–277, Sep. 2013, doi: 10.1016/j.scijus.2013.04.004.
  [41] P. Cormaci, A. Teatino, and A. Barbaro, “Validation of forensic DNA analysis from bloodstains treated by presumptive test reagents,” Int Congr Ser, vol. 1261, no. C, pp. 631–633, 2004, doi: 10.1016/S0531-5131(03)01539-5.
  

  Citation
  Deepthi Nagesh, B. Nagarajamurthy, Bhanuprakash R., "Age Estimation of Concealed and Unconcealed Blood Stains on Different Surfaces – A Chemometric Approach," International Journal of Scientific Research in Chemical Sciences, Vol.11, Issue.4, pp.11-22, 2024

• Open Access   Article

  A study to determine chemical composition of Convolvulus althaeoides L. Plant by using (GC-MS)

  Mayada Mahmoud Zahlout

  Research Paper | Journal-Paper (IJSRCS)

  Vol.11 , Issue.4 , pp.23-27, Aug-2024

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  Abstract
  Plants are an important source of human health, as the pharmaceutical industries today have come to rely on medicinal plants to produce pharmaceutical compounds due to the traditional relationship with them, and the ease of obtaining them and their safety. This study aimed to identify the biochemical compounds which present in flowers, leaves and roots of the species Convolvulus althaeoides L., samples were collected from the Salunfa region of the Lattakia - Syria, during the month of July 2022. The plant extract was obtained using an ultrasound device. It was analyzed by using a chromatography device. Gas spectrometry (GC/MS). 25 compounds were identified in flowers, 14 compounds in roots, and 6 compounds in leaves. The highest percentage of hydrocarbons was in the flowers (25.5%), followed by the roots (7.756%), while a small percentage was recorded in the leaves (2.61%). The two most important compounds were Heptadecan and Eicosane, which have been proven effective as anti-inflammatory in kidney tissue. Fatty acids were found in the flowers (10.26%). ) As for the roots (15.73%) and is absent from the leaves, one of its most important compounds is Palmitic acid. Or called C16, it is an important antimicrobial, anti-hemolytic, antioxidant, and cholesterol-lowering agent. Isopropyl linoleate, which belongs to the ester group, is found to have antioxidant, antimicrobial, and anti-cancer activity. This species is a source of bioactive compounds potentially used in medical applications.

  Key-Words / Index Term
  Convolvulus althaeoides. L, Gas Chromatography (GC/MS), ultrasound device, chemical compounds. Carboxylic acids

  References
  [1] Cronquist, A . An Integrated System of Classification of Flowering Plants. Columbia University Press, NewYork, 1981.
  [2] AL-Alawi. A. Systematic Studies of the Genus Convolvulus L. in the Arabian Peninsula. Reading University, 1987.
  [3] Sa’ad, F . The Convolvulus species of the Canary Isles, the Mediterranean region and the near and Middle East. Mededelingen van het Botanisch Museum en Herbarium van de Riksuniversiteit te Utrecht,Vol. 281, Issue. 1, pp. 3-288, 1967.
  [4] Boulos , L. . Flora of Egypt, (Convolvulacea). Al hadar publishing,Cairo,Egypt, Vol. 2, pp. 352, 2000.
  [5] Mouterde, P. Nouvelle Flore Du Liban et de la Syrie tome II . Dar El-Machreq Éditeurs, Beyrouth, Liban, pp. 334–344, 1986.
  [6] Amrati, FEZ., Bourhia, M., Saghrouchni, H., Sighoua, M., and Grafov, A Caralluma Europaea (Guss.) NE Br. Anti-Inflammatory, Antifungal, and Antibacterial Activities against Nosocomial Antibiotic-Resistant Microbes of Chemically Characterized Fractions. Molecules. Issue. 26, pp. 636, 2021.
  [7] Eastman, J.A. The Book of Field and Roadside: Open Country Weeds, Trees and Wildflowers of Eastern North America, 336 pp., Stackpole Books,Mechanicsburg, Pennsylvania, USA, ISBN: pp. 978, 2015.
  [8] Chen, G.T.; Lu, Y.; Yang, M.; Li, J.-L.; Fan, B.-Y. Medicinal Uses, Pharmacology, and Phytochemistry of Convolvulaceae Plants with Central Nervous System Efficacies: A Systematic Review. Phytother. Res, Vol. 32, pp. 823–864, 2018.
  [9] Elzaawel,Y. A ., and Tawata, S. Antioxidant activity of phenolic rich fraction obtained from Convolvulus arvensis L. leaves grown in Egypt. Asian Journal of Crop Science. pp. 432-40, 2012.
  [10] Kaur, M., & Kalia, A. N. Anticancer potential of the Convolvulus arvensis. International Journal of Pharmaceutical Research & Allied Sciences, Vol. 1, Issue. 3, pp. 101–102, 2012.
  [11] Arora,S., and Kumar,G. Gas Chrom Atography-Mass Specttrometry (GC-MS( Determination Of Bioactive Constituent From The Methanolic And Ethyl Acetate Extract Of Cenchurs Vahl(Poaceae).The Pharma Innovation Jornal,Vol. 6, Issue.11, pp. 635-640, 2017.
  [12] Awaad, A.S., EL-meligy, R.M., Qenawy, S., Atta, A.H., and Soliman, G.A. Anti-inflammatory, antinociceptive and antipyretic effects of some desert plants. Journal of Saudi Chemical Society.Vol. 15, pp. 367-373, 2011.
  [13] Azman, N. A., Gallego, M. G., Julia, L.; Fajari, L., and Almajano, M. The effect of Convolvulus arvensis dried extract as a potential antioxidant in food models. Antioxidants (Basel),Vol. 4, Issue. 1 , pp. 170–184, 2015.
  [14] Mishra, S.H., and Sethiya, N.K. .Review on Ethnomedicinal Uses and Phytopharmacology of Memory Boosting Herb’Convolvulus Pluricaulis’ Choisy. Aust. J. Med. Herbal,Vol. 22, pp. 19–25, 2010.
  [15] Bihaqi, SW., Sharma, Mbourhia, M., Shahat, A., and Almarfadi,m. O. Ethnopharmacological Survey of Herbal Remedies Used for the Treatment of Cancer in the Greater Casablanca-Morocco. Evid.Based Complement. Altern. Med., pp. 1–9, 2019.
  [16] Singh, S., and Vora, S. Studies on alkaloids extraction, identificationby thin layer chromatography from some medicinal plants and evaluating its antimicrobial potential. European Journal of Biomedical and Pharmaceutical Sciences I, Vol. 4, Issue. 6, pp. 627–631, 2017.
  [17] Arora, M., and Malhotra, M. A review on macroscopical, phytochemical and biological studies on Convolvulus arvensis (field bindweed). Pharmacology online.Vol. 3, pp. 1296-1305, 2011.
  [18] Salamatullah, A.M."Convolvulus arvensis: Antioxidant, Antibacterial, and Antifungal Properties of Chemically Profiled Essential Oils: An Approach against Nosocomial Infections. Life, Vol. 12, pp. 2138, 2022.
  [19] Al?Asady, A. A. B., Suker, D. K., and Hassan, K. K. Cytotoxic and cytogenetic effects of Convolvulus arvensis extracts on rhabdomyosarcoma (RD) tumor cell line in vitro. Journal of Medicinal Plant Research, Vol. 8, Issue. 15), pp. 588–598, 2014.
  [20] Hassan, K. K. Cytotoxic and cytogenetic effects of some extracts of Convolvulus arvensis on tumor and transformed cell lines in vitro, 2012.
  [21] Saleem, M., Qadir, M., Ahmad, B., Saleem, D. U., Naseer, F., Schini?Kerth,V., and Hussain, K. Cytotoxic effect of ethanol extract of Convolvulu arvensis L (Convolvulaceae) on lymphoblastic leukemia Jurkat cells. Tropical Journal of Pharmaceutical Research, Vol. 13, pp. 705–709, 2014.
  [22] Hassine, M.; Berguaoui, A., Znati, M., Flamini, G., Ben jannet, H., and Hamza, M. A. Chemical composition, antibacterial and cytotoxic activities of the essential oil from the flowers of Tunisian Convolvulus althaeoides L. Natural Product Research, Vol. 28, Issue. 11, pp. 769–775, 2014.
  [23] Starlin T, Prabha PS, Thayakumar BKA, Gopalakrishnan VK. Screening and GC-MS profiling of ethanolic extract of Tylophora pauciflora. Biomed Inform. Vol. 15, Issue. 6, pp.425–429, 2019.
  [24] Schauenberg, P .Guide des plantes médicinales, anlyse, description et utilisation de 400 plantes. Ed. Delachaux et niestlé , 2006.
  [25] Stanisavljevic ,I.T., Lazic, M.L., and Veljkovic ,V.B. Ultrasonic extraction of oil from tobacco (Nicotiana tabacum L.) seeds. Ultrason Sonochem .Vol. 14, pp.646–652, 2007.
  [26] Merlin NJ, Parthasarathy V, Manavala R and Kumaravel S: Chemical investigation of aerial parts of Gmelina asiatica Linn by GC-MS. Pharm Res; Vol. 1, Issue. 3, pp. 152-56, 2009.
  [27] Sunish, K., and Dhivya, R. GC-MS Analysis and Antioxidant Activity of Organic Extracts of Psidium cattleianum Leaves. International Journal of Scientific Research in Biological Sciences. Vol. 7, Issue. 2, pp. 128-133, 2020.
  [28] Chelliah, R., Ramakrishnan, S., and Antony,U..Nutritional quality of Moringa oleifera for its bioactivity and antibacterial properties. International Food Research Journal, Vol. 24, Issue. 2, pp. 825, 2017.
  [29] Konovalova,O., Gergel,E., and Herhel,V. GC-MS ANALYSIS of bioactive components of Shepherdia argentea (pursh) nutt. From Ukrainian Flora.the pharma innovation jornal,Vol .2, Issue. 6, pp. 125-140, 2013.
  [30] AL-Ibrahemi .N, AL-Yasssiry.A AL-Laith,Z, and AL-Musawi.B. (Phytochemical Study of Volatile Oils for the Ocimum basilicum L. and Mentha spicata By Gas Chromatography .OP Conf. Ser.: Earth Environ, 2023.
  [31] Rajendran, P., Bharathidasan ,R, ., and Sureshkumar, K. GC-MS analysis of phyto-components in raw and treated sugarcane juice. Int J Curr Microbiol Appl Sci; Vol. 6, pp. 51-61, 2017.
  [32] Hrichi, S., Chaâbane-banaoues, R, Alibrando, F., Altemimi, A.B., Babba, O., and Majdoub, Y.O.E. Chemical Composition, Antifungal and Anti-Biofilm Activities ofVolatile Fractions of Convolvulus Althaeoides L. Roots from Tunisia. Molecules, Vol. 27, pp. 6834, 2022.
  [33] Inikpi, E., Lawal, O. A., Ogunmoye, A.O., and Ogunwande, I. A. Volatile composition of the floral essential oil of Hibiscus sabdariffa L. from Nigeria . American Journal of Essential Oils and Natural Products; Vol. 2, Issue. 2, 04-07, 2014.
  [34] Obouayeba, A. P., Okoma, K. M., Diarrasouba, M., Diabaté, S., and Kouakou, T. HPhytochemical Characterisation and Antioxidant Activity". Vol. 20, pp. 14-20, 2015.
  [35] Ashraf, I., Zubair, M., Rizwan, K., Rasool, N., Jamil, M., Khan, S.A.; and Tareen, R.B. Chemical composition, antioxidant and antimicrobial potential of essential oils from different parts of Daphne mucronata Royle. Chem Cent J. Dec. Vol. 17, Issue. 12, pp. 135, 2018.
  [36] Ceballos, D.M., Fellows, K.M., Evans, A.E, Janulewicz, P.A., Lee, E.G., and Whittaker, S.G. Perchloroethylene and Dry Cleaning: It`s Time to Move the Industry to Safer Alternatives. Front. Public Health, Issue. 9, 2021.

  Citation
  Mayada Mahmoud Zahlout, "A study to determine chemical composition of Convolvulus althaeoides L. Plant by using (GC-MS)," International Journal of Scientific Research in Chemical Sciences, Vol.11, Issue.4, pp.23-27, 2024